The structurally distinct FMN-dependent nitroreductase-like (NTR) superfamily is comprised of over 25,000 enzymes that are typically organized as dimers with two discrete active sites located at the dimer interface. Named for its initially characterized nitroreductase reaction, the superfamily is now known to catalyze a broad range of reactions associated with many different types of compounds. The canonical reaction involves the oxidation of an electron donor, such as NAD(P)H and the ensuing reduction of an electron acceptor, e.g., a nitroaromatic compound, which occurs via an electron shuttle involving flavin mononucleotide (FMN). As with many functionally diverse superfamilies, the huge majority of their sequences are of unknown function. The classification paper “Evolutionary and molecular foundations of multiple contemporary functions of the nitroreductase superfamily” (PMID 29078300) parses the superfamily into subgroups based on sequence similarity and other features. Only 14 of the 22 major subgroups resulting from this classification can be associated with a biochemically characterized enzyme. Sequence similarity networks and annotation data for all of these subgroups are available from the “Download Archived Data” tab. Similarity networks are also provided for figure 2 and supplementary figure S5, colored and highlighted as they are shown in this paper.

The NTR superfamily was also used as the model for the publication, “Revealing Unexplored Sequence-Function Space Using Sequence Similarity Networks” (PMID 30052428). Data files described in this paper are available from the “Download Archived Data” tab as well.

No notes.

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