Top Level Name

  ⌊ Superfamily (core) Enolase

Family known
Total 100% <100% Family unknown
Functional domains 48847 0 31936 16911
UniProtKB 94498 0 71915 22583
GI 189099 0 137582 51517
Structures 371
Reactions 33
Functional domains of this superfamily were last updated on Nov. 22, 2017
New functional domains were last added to this superfamily on Sept. 13, 2017

Enzymes in the enolase superfamily are related by their ability to catalyze the abstraction of a proton alpha to a carboxylic acid to form an enolate anion intermediate. This conserved partial reaction is mediated by conserved residues in the active site, including three residues used to bind a divalent metal involved in stabilization of the common intermediate.

Reactions catalyzed by these enzymes include racemization, beta-elimination of OH- or NH3, and cycloisomerization. Each member has two structural domains, an N-terminal “capping” domain and a C-terminal beta8/alpha8-barrel domain, both of which are required for function. In general, the catalytic residues can be found at ends of the beta strands in the C-terminal barrel domain, while the residues required for substrate specificity are contained in the N-terminal domain. Although the beta8/alpha8-barrel fold is common to many other superfamilies, none of these superfamilies show a significant level of sequence or functional similarity to the enolase superfamily.

Networks curated for the enolase SF in the SFLD are available for download via the Download Archived Data tab. An additional set of networks that include structure and sequence similarity networks, chemical similarity networks, and additional networks from the MEERCat infrastructure paper are available for download here. The MEERCat paper links enzyme reactions and ligands to their associated proteins using the Enolase superfamily as a detailed example.

Babbitt, P.C., et al.

The enolase superfamily: a general strategy for enzyme-catalyzed abstraction of the alpha-protons of carboxylic acids

▸ Abstract

Biochemistry 1996;35(51):16489-16501 | PubMed ID: 8987982

EFI

abstraction of proton alpha to carboxylate | Evidence Code: IDA

Static File Downloads

File Name Description Parameters Stats
repnet.sf1.th50.pE20.mek250.xgmml Representative network: each node is a group of similar sequences node similarity threshold = 50
max edge count = 250
min -log10 E = 20
size = 91M
num_edges = 250000
num_nodes = 1828
sfld_superfamily_1.tsv Annotation data table, tab separated columns size=17M
#rows=48851
sfld_superfamily_1.fasta Protein sequences, fasta format size=21M
#seqs=48850
sfld_molecule_network_superfamily_1.xgmml molecule network, all size=323K
num_edges=1608
num_nodes=62
sfld_molecule_product_network_superfamily_1.xgmml molecule network, product size=118K
num_edges=415
num_nodes=34
sfld_molecule_substrate_network_superfamily_1.xgmml molecule network, substrate size=103K
num_edges=442
num_nodes=31
sfld_reaction_network_superfamily_1.xgmml reaction network, size=235K
num_edges=528
num_nodes=33
sfldAlignmentSF1.msa Annotated Sequence Alignment, Stockholm format 33 sequences
size: 29K

Total number of functional domains in this group.
Number of Functional Domains that have been manually or automatically been assigned to a family.
Number of Functional Domains that have not been assigned to a family.
Number of structures available from the PDB for members of this group.
Number of Functional Domains with 100% of Conserved Residues
Number of Functional Domains with less than 100% Conserved Residues
Subgroup ▸ Legend T K C U S
enolase 13877 13530 0 347 70
┗ enolase 13530 13530 0 60
galactarate dehydratase 25 2 0 23 6
┗ galactarate dehydratase 2 2 2 0 6
glucarate dehydratase 4012 3585 0 427 30
┗ glucarate dehydratase 3585 3585 0 22
mandelate racemase 12476 5059 0 7417 117
┗ 3,6-anhydro-alpha-L-galactonate cycloisomerase 108 108 0 0
┗ D-arabinonate dehydratase 10 10 0 0
┗ D-galactonate dehydratase 1581 1581 0 2
┗ D-tartrate dehydratase 290 290 0 3
┗ galactarate dehydratase 3 96 96 0 0
┗ galactarolactone cycloisomerase 53 53 0 0
┗ gluconate dehydratase 13 13 0 0
┗ L-fuconate dehydratase 1191 1191 0 7
┗ L-galactonate dehydratase 229 229 0 0
┗ L-lyxonate dehydratase 245 245 0 0
┗ L-talarate/galactarate dehydratase 319 319 0 5
┗ mandelate racemase 11 11 0 11
┗ rhamnonate dehydratase 769 769 0 11
┗ xylonate dehydratase 1 10 10 0 0
┗ xylonate dehydratase 2 134 134 0 0
mannonate dehydratase 1843 169 0 1674 39
┗ mannonate dehydratase 169 169 0 24
methylaspartate ammonia-lyase 305 154 0 151 6
┗ methylaspartate ammonia-lyase 154 154 0 6
muconate cycloisomerase 13427 9437 0 3990 103
┗ 4R-hydroxyproline betaine 2-epimerase 79 79 0 0
┗ cis-3-hydroxy-L-proline dehydratase 29 29 0 0
┗ dipeptide epimerase 4657 4657 0 29
┗ muconate cycloisomerase (anti) 113 113 0 3
┗ N-succinylamino acid racemase 1 17 17 0 9
┗ N-succinylamino acid racemase 2 237 237 0 3
┗ o-succinylbenzoate synthase 4305 4305 0 27
┗ 1: muconate cycloisomerase (syn) like 1525 0 0 1525 13
Depth of the multi-level Subgroup hierarchy.