Top Level Name

  ⌊ Superfamily (core) Enolase

    ⌊ Subgroup mannonate dehydratase

Family known
Total 100% <100% Family unknown
Functional domains 1843 0 169 1674
UniProtKB 5389 0 118 5271
GI 10985 0 345 10640
Structures 39
Reactions 0
Functional domains of this subgroup were last updated on Nov. 22, 2017
New functional domains were last added to this subgroup on June 8, 2015

The mannonate dehydratase (manD) group consists of the subset of enolase superfamily enzymes with a conserved tyrosine, presumably acting as an active site base, at the position of Y159 in the experimentally characterized manD from Novosphingobium aromaticivorans. An additional conserved arginine controls the pKa of this tyrosine (R147 in N. aromaticivorans manD). At the present time, mannonate dehydratase is the only characterized family within this group. Other enzymes within the group have been shown to catalyze the low efficiency dehydration of D-gluconate and/or D-mannonate, but the biologically relevant function of these enzymes is unclear.

Rakus JF, Fedorov AA, Fedorov EV, Glasner ME, Vick JE, Babbitt PC, Almo SC, Gerlt JA

Evolution of Enzymatic Activities in the Enolase Superfamily: d-Mannonate Dehydratase from Novosphingobium aromaticivorans

▸ Abstract

Biochemistry 2007;46(45):12896-12908 | PubMed ID: 17944491

Wichelecki DJ, Balthazor BM, Chau AC, Vetting MW, Fedorov AA, Fedorov EV, Lukk T, Patskovsky YV, Stead MB, Hillerich BS, Seidel RD, Almo SC, Gerlt JA

Discovery of function in the enolase superfamily: D-mannonate and d-gluconate dehydratases in the D-mannonate dehydratase subgroup

▸ Abstract

Biochemistry 2014;53(16):2722-2731 | PubMed ID: 24697546

Wichelecki DJ, Vendiola JA, Jones AM, Al-Obaidi N, Almo SC, Gerlt JA

Investigating the physiological roles of low-efficiency D-mannonate and D-gluconate dehydratases in the enolase superfamily: pathways for the catabolism of L-gulonate and L-idonate

▸ Abstract

Biochemistry 2014;53(35):5692-5699 | PubMed ID: 25145794

No notes.

Static File Downloads

File Name Description Parameters Stats
network.sg33.bs60.mek250K.xgmml One node per sequence network min bit score = 60
max edge count = 250K
size = 84M
num_edges = 250000
num_nodes = 1844
repnet.sg33.th50.pE20.mek250.xgmml Representative network: each node is a group of similar sequences node similarity threshold = 50
max edge count = 250
min -log10 E = 20
size = 986K
num_edges = 120
num_nodes = 16
sfld_alignment_sg33.msa Annotated Sequence Alignment, Stockholm format 47 sequences
size: 24K

Total number of functional domains in this group.
Number of Functional Domains that have been manually or automatically been assigned to a family.
Number of Functional Domains that have not been assigned to a family.
Number of structures available from the PDB for members of this group.
Number of Functional Domains with 100% of Conserved Residues
Number of Functional Domains with less than 100% Conserved Residues
Subgroup ▸ Legend T K C U S
mannonate dehydratase 1843 169 0 1674 39
┗ mannonate dehydratase 169 169 0 24
Depth of the multi-level Subgroup hierarchy.