Top Level Name

  ⌊ Superfamily (core) Enolase

    ⌊ Subgroup galactarate dehydratase

  ⌊ FunctionalDomain Uncharacterized Galactarate dehydratase subgroup sequence (ID 92485)

Superfamily Assignment Evidence Code(s) ISS
This entry was last updated onFeb. 7, 2017

References to Other Databases

Genbank

SpeciesGIAccessionProteome
Oceanobacillus iheyensis HTE831 Taxon ID: 221109 281500775 URP
Oceanobacillus iheyensis HTE831 Taxon ID: 221109 281500774 URP

Uniprot

Protein NameAccessionEC Number Identifier
Galactarate dehydratase (D-threo-forming) Q8EMJ9 GALRD_OCEIH (Swiss-Prot)

Sequence

Length of Enzyme (full-length): 391 | Length of Functional Domain: 391

1       10        20        30        40        50        60

MKITDLELHAVGIPRHTGFVNKHVIVKIHTDEGLTGIGEMSDFSHLPLYSVDLHDLKQGL
LSILLGQNPFDLMKINKELTDNFPETMYYFEKGSFIRNGIDNALHDLCAKYLDISVSDFL
GGRVKEKIKVCYPIFRHRFSEEVESNLDVVRQKLEQGFDVFRLYVGKNLDADEEFLSRVK
EEFGSRVRIKSYDFSHLLNWKDAHRAIKRLTKYDLGLEMIESPAPRNDFDGLYQLRLKTD
YPISEHVWSFKQQQEMIKKDAIDIFNISPVFIGGLTSAKKAAYAAEVASKDVVLGTTQEL
SVGTAAMAHLGCSLTNINHTSDPTGPELYVGDVVKNRVTYKDGYLYAPDRSVKGLGIELD
ESLLAKYQVPDLSWDNVTVHQLQDRTADTKS
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Conserved Residues
Superfamily CAR This EFD conserves 3/3 Superfamily-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
193 Asp (D) side chain metal binding ligand metal ligand -- binding ISS PubMed:8987982
221 Glu (E) side chain metal binding ligand metal ligand -- binding ISS PubMed:8987982
245 Glu (E) side chain metal binding ligand metal ligand -- binding ISS PubMed:8987982
Subgroup CAR This EFD conserves 8/9 Subgroup-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
42 Asp (D) side chain metal binding ligand (second metal) metal ligand -- binding ICS PubMed:19883118
45 His (H) side chain metal binding ligand (second metal) metal ligand -- binding ICS PubMed:19883118
90 Phe (F) side chain MISMATCH: This residue does not match the specified amino acid type of Y, and thus may not function in the same manner as other sequences in the subgroup
162 Arg (R) side chain controls pKa of Tyr perturbates pKa -- spectator ICS PubMed:19883118
164 Tyr (Y) side chain abstracts proton from C2 of galactarate proton relay -- reactant ICS PubMed:19883118
193 Asp (D) side chain metal binding ligand metal ligand -- binding ICS PubMed:19883118
221 Glu (E) side chain metal binding ligand metal ligand -- binding ICS PubMed:19883118
246 His (H) side chain metal binding ligand metal ligand -- binding ICS PubMed:19883118
297 Thr (T) side chain metal binding ligand (second metal) metal ligand -- binding ICS PubMed:19883118

Structures

PDB ID Title Molecule Name Number of
EFDs		 Resolution (Å) Mutant? Het group Links
3HPF Crystal Structure Of The Mutant Y90F Of Divergent Galactarate Dehydratase From Oceanobacillus Iheyensis Complexed With Mg And Galactarate Muconate Cycloisomerase 4 1.8 Yes D-Galactaric Acid • Magnesium Ion CSA • PDB • PDBSum
3GD6 Crystal Structure Of Divergent Enolase From Oceanobacillus Iheyensis Complexed With Phosphate Muconate Cycloisomerase 4 1.6 Phosphate Ion CSA • PDB • PDBSum
3ES8 Crystal Structure Of Divergent Enolase From Oceanobacillus Iheyensis Complexed With Mg And L-Malate. Muconate Cycloisomerase 4 2.2 (2S)-2-Hydroxybutanedioic Acid • Magnesium Ion CSA • PDB • PDBSum
2OQY The Crystal Structure Of Muconate Cycloisomerase From Oceanobacillus Iheyensis Muconate Cycloisomerase 4 2.0 Magnesium Ion CSA • PDB • PDBSum
3ES7 Crystal Structure Of Divergent Enolase From Oceanobacillus Iheyensis Complexed With Mg And L-Malate. Muconate Cycloisomerase 4 1.9 (2S)-2-Hydroxybutanedioic Acid • Magnesium Ion CSA • PDB • PDBSum
3FYY Crystal Structure Of Divergent Enolase From Oceanobacillus Iheyensis Complexed With Mg Muconate Cycloisomerase 4 1.8 Magnesium Ion CSA • PDB • PDBSum
Percent identity and alignment length from the BLAST match of the Functional Domain sequence to the PDB sequence.
"Yes" indicates that a GI associated with this Functional Domain was mapped to the PDB ID via UniProtKB.

Curation History
Time Change Annotation Old Value New Value
Aug. 1, 2014, 2:44 a.m. update curation agent sbrown setDomainBoundaries.py
EC number assigned by UniProtKB accession ID.