Top Level Name

  ⌊ Superfamily (core) Enolase

    ⌊ Subgroup muconate cycloisomerase

  ⌊ muconate cycloisomerase (syn) like

  ⌊ FunctionalDomain Uncharacterized (chloro)muconate cycloisomerase (syn) like subgroup sequence (ID 6040)

Superfamily Assignment Evidence Code(s) ISS
This entry was last updated onNov. 22, 2017

References to Other Databases

Genbank

SpeciesGIAccessionProteome
Pseudomonas fluorescens CHA0 Taxon ID: 1124983 500243528 AGL85686.1 (Genbank) URP
Pseudomonas fluorescens Pf-5 Taxon ID: 220664 68345520 AAY93126.1 (Genbank) URP

Uniprot

Protein NameAccessionEC Number Identifier
n/a Q4K9X1 Q4K9X1_PSEF5 (TrEMBL)

Sequence

Length of Enzyme (full-length): 382 | Length of Functional Domain: 374

1       10        20        30        40        50        60

MHASAIESIETIIVDLPTIRPHKLAMHTMQNQTLVLIRLRCADGIEGLGESTTIGGLAYG
NESPDSIKTNIDRFVAPLLIGQDASNINAAMLRLEQSIRGNTFAKSGIESALLDAQGKRL
GLPVSELLGGRVRDALPVAWTLASGDTAKDIAEAQKMLDLRRHRIFKLKIGAGEVDRDLA
HVIAIKKALGDSASVRVDVNQAWDEAVALRACRILGGNGIDLIEQPISRNNRAGMVRLNA
SSPAPIMADESIECVEDAFNLAREGAASVFALKIAKNGGPRATLRTAAIAEAAGIGLYGG
TMLEGGIGTLASAHAFLTLNKLSWDTELFGPLLLTEDILAEPPVYRDFHLHVSKAPGLGL
SLDEERLAFFRRDKTTPVLHHT
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Conserved Residues
Superfamily CAR This EFD conserves 3/3 Superfamily-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
198 Asp (D) side chain metal binding ligand metal ligand -- binding ISS PubMed:8987982
224 Glu (E) side chain metal binding ligand metal ligand -- binding ISS PubMed:8987982
249 Asp (D) side chain metal binding ligand metal ligand -- binding ISS PubMed:8987982
Subgroup CAR This EFD conserves 7/7 Subgroup-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
167 Lys (K) side chain Electrostatic stabilization of transition state electrostatic stabiliser -- spectator ISS PubMed:10336378
169 Lys (K) side chain abstracts alpha proton (base) proton relay -- reactant ICS PubMed:10336378
198 Asp (D) side chain metal binding ligand metal ligand -- binding ICS PubMed:9724714
224 Glu (E) side chain metal binding ligand metal ligand -- binding ICS PubMed:9724714
249 Asp (D) side chain metal binding ligand metal ligand -- binding ICS PubMed:9724714
273 Lys (K) side chain Stabilizes enolate anion intermediate electrostatic stabiliser -- spectator ICS PubMed:19220063
327 Glu (E) side chain general acid proton relay -- reactant ICS PubMed:7500361

Structures

PDB ID Title Molecule Name Number of
EFDs		 Resolution (Å) Mutant? Het group Links
3CT2 Crystal Structure Of Muconate Cycloisomerase From Pseudomonas Fluorescens Muconate Cycloisomerase 10 1.8 Magnesium Ion CSA • PDB • PDBSum
3FJ4 Crystal Structure Of Muconate Lactonizing Enzyme From Pseudomonas Fluorescens Complexed With Muconolactone Muconate Cycloisomerase 10 1.8 [(2S)-5-Oxo-2,5-Dihydrofuran-2-Yl]Acetic Acid • Magnesium Ion CSA • PDB • PDBSum
3DGB Crystal Structure Of Muconate Lactonizing Enzyme From Pseudomonas Fluorescens Complexed With Muconolactone Muconate Cycloisomerase 10 1.7 Magnesium Ion • [(2S)-5-Oxo-2,5-Dihydrofuran-2-Yl]Acetic Acid CSA • PDB • PDBSum
Percent identity and alignment length from the BLAST match of the Functional Domain sequence to the PDB sequence.
"Yes" indicates that a GI associated with this Functional Domain was mapped to the PDB ID via UniProtKB.

Curation History
Time Change Annotation Old Value New Value
Nov. 3, 2014, 2:34 a.m. update curation agent updateSFLD2.py setDomainBoundaries.py
Aug. 10, 2015, 3:03 a.m. update domain end position 373 374
Oct. 15, 2015, 3:27 a.m. update curation agent setDomainBoundaries.py sbrown
remove family assignment evidence code IEA -
remove family muconate cycloisomerase (syn) -
update name muconate cycloisomerase (syn) Uncharacterized (chloro)muconate cycloisomerase (syn) like subgroup sequence
update subgroup muconate cycloisomerase muconate cycloisomerase (syn) like
update superfamily assignment evidence code IEA ISS
Feb. 10, 2017, 3:04 a.m. update curation agent sbrown setDomainBoundaries.py
EC number assigned by UniProtKB accession ID.