Top Level Name

  ⌊ Superfamily (core) Enolase

    ⌊ Subgroup muconate cycloisomerase

  ⌊ muconate cycloisomerase (syn) like

  ⌊ FunctionalDomain 2,4-dichloromuconate cycloisomerase (ID 5886)

Superfamily Assignment Evidence Code(s) ISS
This entry was last updated onNov. 22, 2017

References to Other Databases

Genbank

SpeciesGIAccessionProteome
Sphingobium herbicidovorans NBRC 16415 Taxon ID: 1219045 672387053 KFG88791.1 (Genbank) URP
Sphingomonas sp. TFD44 Taxon ID: 77201 51243151 AAT99367.1 (Genbank)

Uniprot

Protein NameAccessionEC Number Identifier
n/a Q5YBA1 Q5YBA1_9SPHN (TrEMBL)
n/a A0A086P5X3 A0A086P5X3_SPHHM (TrEMBL)

Sequence

Length of Enzyme (full-length): 398 | Length of Functional Domain: 380

1       10        20        30        40        50        60

MTLVTELTPPAASAPPFSDHPDHVIAAVTATIVDLPLRRPHFHATGTHATQSLVMVELVT
SGGAVGWGEGGTPGGTAFWGGECVETIKLMIDHYLAPAVIGISVFDQERILAAMDRIAAN
NHFAKAAIDIAVHDAAARLIGISVSQMFGGTARSTVPVLWALAAAAYEADVADAIEQIEA
GRHSVFKIKIGKGDAASETRRALETATTIHDAHPDARFVVDLNQAWDESTAMRWLAAFQE
RDFLFVEQPVPAWNVEGMIRLASRFEIPIMADEGLWDFHDAYELIRRGATDIYAVKIAKG
GGLRRAFKAAAVAEAAGVPLYGGMALESSVGTAAGLQLFSALPNLPFGCELIGPRLLAED
LTVQPIAYRDREVVVPSGVGMGVEIDRDRVRSFTRRNK
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Conserved Residues
Superfamily CAR This EFD conserves 3/3 Superfamily-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
221 Asp (D) side chain metal binding ligand metal ligand -- binding ISS PubMed:8987982
247 Glu (E) side chain metal binding ligand metal ligand -- binding ISS PubMed:8987982
273 Glu (E) side chain metal binding ligand metal ligand -- binding ISS PubMed:8987982
Subgroup CAR This EFD conserves 7/7 Subgroup-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
187 Lys (K) side chain Electrostatic stabilization of transition state electrostatic stabiliser -- spectator ISS PubMed:10336378
189 Lys (K) side chain abstracts alpha proton (base) proton relay -- reactant ICS PubMed:10336378
221 Asp (D) side chain metal binding ligand metal ligand -- binding ICS PubMed:9724714
247 Glu (E) side chain metal binding ligand metal ligand -- binding ICS PubMed:9724714
272 Asp (D) side chain metal binding ligand metal ligand -- binding ICS PubMed:9724714
296 Lys (K) side chain Stabilizes enolate anion intermediate electrostatic stabiliser -- spectator ICS PubMed:19220063
350 Glu (E) side chain general acid proton relay -- reactant ICS PubMed:7500361

Catalyzed Reaction

2,4-dichloromuconate cycloisomerase

+
2,4-dichloro-cis,cis-muconate(2-)
11438		 cis-2-chloro-4-carboxylatomethylenebut-2-en-1,4-olide
57681		 hydrogen chloride
17883

EC: 5.5.1.11 | IntEnz: 5.5.1.11 | Kegg: 5.5.1.11 | BioCyc: 5.5.1.11 | BRENDA: 5.5.1.11

Curation History
Time Change Annotation Old Value New Value
Nov. 3, 2014, 2:34 a.m. update curation agent updateSFLD2.py setDomainBoundaries.py
Aug. 10, 2015, 3:03 a.m. update domain start position 23 19
Oct. 15, 2015, 3:27 a.m. update curation agent setDomainBoundaries.py sbrown
update curation agent sbrown setDomainBoundaries.py
update domain end position 397 398
remove family assignment evidence code IEA -
remove family chloromuconate cycloisomerase -
update name chloromuconate cycloisomerase 2,4-dichloromuconate cycloisomerase
update subgroup muconate cycloisomerase muconate cycloisomerase (syn) like
update superfamily assignment evidence code IEA ISS
EC number assigned by UniProtKB accession ID.