Top Level Name

  ⌊ Superfamily (core) Radical SAM

    ⌊ Subgroup DesII-like

     ⌊ Family dTDP-4-amino-4,6-dideoxy-D-glucose ammonia-lyase (DesII-like)

  ⌊ FunctionalDomain DesII (ID 419744)

Superfamily Assignment Evidence Code(s) ISS PubMed:21513273
Family Assignment Evidence Code CFM PubMed:21513273 PubMed:19746907 PubMed:16187386 PubMed:20121093
This entry was last updated onJune 10, 2017

References to Other Databases

Genbank

SpeciesGIAccessionProteome
n/a 67620657 AAY77315.1 (Genbank)
n/a 29711438 AAO98873.1 (Genbank)
n/a 29703233 AAO96662.1 (Genbank)
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Uniprot

Protein NameAccessionEC Number Identifier
dTDP-4-amino-4,6-dideoxy-D-glucose ammonia-lyase {ECO:0000303|PubMed:19746907} Q9ZGH1 DESII_STRVZ (Swiss-Prot)

Sequence

Length of Enzyme (full-length): 485 | Length of Functional Domain: 485

1       10        20        30        40        50        60

MTAPALSATAPAERCAHPGADLGAAVHAVGQTLAAGGLVPPDEAGTTARHLVRLAVRYGN
SPFTPLEEARHDLGVDRDAFRRLLALFGQVPELRTAVETGPAGAYWKNTLLPLEQRGVFD
AALARKPVFPYSVGLYPGPTCMFRCHFCVRVTGARYDPSALDAGNAMFRSVIDEIPAGNP
SAMYFSGGLEPLTNPGLGSLAAHATDHGLRPTVYTNSFALTERTLERQPGLWGLHAIRTS
LYGLNDEEYEQTTGKKAAFRRVRENLRRFQQLRAERESPINLGFAYIVLPGRASRLLDLV
DFIADLNDAGQGRTIDFVNIREDYSGRDDGKLPQEERAELQEALNAFEERVRERTPGLHI
DYGYALNSLRTGADAELLRIKPATMRPTAHPQVAVQVDLLGDVYLYREAGFPDLDGATRY
IAGRVTPDTSLTEVVRDFVERGGEVAAVDGDEYFMDGFDQVVTARLNQLERDAADGWEEA
RGFLR
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Conserved Residues
Superfamily CAR This EFD conserves 3/3 Superfamily-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
141 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
145 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
148 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
Subgroup CAR This EFD conserves 3/3 Subgroup-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
141 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IEA
145 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IEA
148 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IEA
Family CAR This EFD conserves 3/3 Family-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
141 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IEA
145 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IEA
148 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IEA

Catalyzed Reaction

dTDP-4-amino-4,6-dideoxy-D-glucose ammonia-lyase

+ + + + + + +
dTDP-4-amino-4,6-dideoxy-alpha-D-glucose(1-)
68501		 S-adenosyl-L-methionine zwitterion
59789		 hydrogen donor
17499		 dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucose(2-)
76280		 5'-deoxyadenosine
17319		 L-methionine zwitterion
57844		 hydrogen acceptor
13193		 hydron
15378		 ammonium
28938

EC: 4.3.1.30 | IntEnz: 4.3.1.30 | Kegg: 4.3.1.30 | BioCyc: 4.3.1.30 | BRENDA: 4.3.1.30

Curation History
Time Change Annotation Old Value New Value
May 14, 2014, 3:08 a.m. update curation agent holliday setDomainBoundaries.py
update domain end position 307 485
update domain start position 128 1
July 15, 2014, 6:19 a.m. update curation agent setDomainBoundaries.py holliday
Oct. 16, 2014, 6:47 a.m. update curation agent holliday setDomainBoundaries.py
May 11, 2015, 1:52 p.m. update curation agent setDomainBoundaries.py holliday
update curation agent holliday setDomainBoundaries.py
EC number assigned by UniProtKB accession ID.