Top Level Name

  ⌊ Superfamily (core) Radical SAM

    ⌊ Subgroup B12-binding domain containing

  ⌊ methyltransferase (Class B)

     ⌊ Family 5''-pyrrole methytransferase

  ⌊ FunctionalDomain clorobiocin pyrrole-2-carboxy methyltransferase, CloN6 (ID 412925)

Superfamily Assignment Evidence Code(s) ISS PubMed:12898629
Family Assignment Evidence Code CFM PubMed:12898629
This entry was last updated onJune 10, 2017

References to Other Databases

Genbank

SpeciesGIAccessionProteome
Streptomyces roseochromogenus Taxon ID: 285450 558890409 WP_023545102.1 (RefSeq)
Streptomyces roseochromogenes subsp. oscitans DS 12.976 Taxon ID: 1352936 558544644 EST35516.1 (Genbank) URP
Streptomyces roseochromogenes subsp. oscitans DS 12.976 Taxon ID: 1352936 24940608 AAN65235.1 (Genbank) URP

Uniprot

Protein NameAccessionEC Number Identifier
n/a Q8GHB6 Q8GHB6_STRRC (TrEMBL)
n/a V6KVQ0 V6KVQ0_STRRC (TrEMBL)

Sequence

Length of Enzyme (full-length): 561 | Length of Functional Domain: 559

1       10        20        30        40        50        60

MFLLHAPSVYDFRDRDDILFAYLSDSDSVNVTSVYEMYPIGWFSIKQQLADHDFDCQIVN
IASLMLKHPDLDVERLIERLEAPVFGFDLHWMTQCNGAIELAEVVKRIHPESLIVFGGIS
ATYFARELIQYPQVDVVVQGYDTLAPITQLVEAVVRGGRDFSSIPNLLYKGNDRVETTGF
SHKPATDYNNVRNDWSYYQNTPVTPLASKLIMTLPNTGCAHDCGWCGGSRFAYRNIMEVG
TKTLVQKDNDLIVEELKTMGEAAKHTSIYALQCYSEGKARMHTYLDAVHDLGYRSVFFEQ
FNLTPHDTLKKMGTSTDSYIMLSPESHDPKISRAAGRGTYTMAEMEEWIPRALDAGVKGV
MIWFFIGMPYQDRQSVRDTVTYSEHLIRKFAGQPVLPLICPMVPFLDPGCRFFEEPDKHG
YRIHHRTLEEHRQAMVEPLWYRRLNYETKWLSRRGIQDVSYEAIERLVSIKGEHGVLSSV
FCEAVLKTIDQTKRLLAEMDHALSLDGRLPATLRDEMRAYNRKILAYSSDQILPIPRPFG
GRWFDDVTVPEEMIRELSMTP
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Conserved Residues
Superfamily CAR This EFD conserves 3/3 Superfamily-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
219 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
223 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
226 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
Subgroup CAR This EFD conserves 3/3 Subgroup-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
219 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
223 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
226 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
Family CAR This EFD conserves 3/3 Family-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
219 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IEA
223 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IEA
226 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IEA

Catalyzed Reaction

clorobiocin pyrrole-2-carboxy methyltransferase

+ 2 + + +
novclobiocin 109
132424		 S-adenosyl-L-methionine zwitterion
59789		 clorobiocin
47302		 5'-deoxyadenosine
17319		 L-methionine zwitterion
57844		 S-adenosyl-L-homocysteine zwitterion
57856

EC: | IntEnz: | Kegg: | BioCyc: | BRENDA: |

Curation History
Time Change Annotation Old Value New Value
May 14, 2014, 3:03 a.m. update curation agent holliday setDomainBoundaries.py
update domain end position 437 559
update domain start position 208 1
July 15, 2014, 6:05 a.m. update classification public staff
update curation agent setDomainBoundaries.py holliday
update domain end position 559 561
July 15, 2014, 6:05 a.m. update curation agent holliday setDomainBoundaries.py
update domain end position 561 559
Aug. 15, 2014, 5:12 a.m. update classification staff public
update curation agent setDomainBoundaries.py holliday
Oct. 16, 2014, 6:26 a.m. update curation agent holliday setDomainBoundaries.py
Oct. 15, 2016, 6:13 a.m. update curation agent setDomainBoundaries.py holliday
update curation agent holliday setDomainBoundaries.py
update name CloN6 clorobiocin pyrrole-2-carboxy methyltransferase, CloN6
EC number assigned by UniProtKB accession ID.