Top Level Name

  ⌊ Superfamily (core) Radical SAM

    ⌊ Subgroup B12-binding domain containing

  ⌊ methyltransferase (Class B)

     ⌊ Family gentamicin-like N-methyltransferase (GntE-like)

  ⌊ FunctionalDomain Gentamycin oxidoreductase (GntE) (ID 412696)

Superfamily Assignment Evidence Code(s) ISS
Family Assignment Evidence Code CFM PubMed:18533111
This entry was last updated onJune 10, 2017

References to Other Databases

Genbank

SpeciesGIAccessionProteome
Micromonospora echinospora Taxon ID: 1877 41019293 AAR98551.1 (Genbank)

Uniprot

Protein NameAccessionEC Number Identifier
n/a Q6QVU0 Q6QVU0_MICEC (TrEMBL)

Sequence

Length of Enzyme (full-length): 634 | Length of Functional Domain: 634

1       10        20        30        40        50        60

MATLTAYLRDKGMPAVGLDLNADFNEYLLNRVEIEQVQGPENTHEFTKPFIKQFFLNHIT
GNYFTETNFEQWDLQQQCQVAPESLSIWDPPFPFSYCEFLSILRDEPERVAKLVRDPDAN
IYHAFYQEKVAGKASELGLMGFSIMGYNQVIPALTLGYLMKKENPDLYICWGGPWVTSFA
DMLIPRLEACPELGELIDALVVREGEEPLLKMAEALSRGERPVGIPGGCKPMSEQSVLDT
KPVGRKLLLAMSQPRENMPNTHWRIEDGTYERSGDISWVADMNQLPTPDYSDFDLSLYTT
FREGQGSLVLQGSRSCYYMKCSFCNAITNFAPWSYRERSTENIQKDIDTFLELYPGTVHF
DFADAVFPAKRLVELADFFIAKKRPELFWEVDVRFEGNIDKAVLTKMRDSQGTLRFGLET
ANERLLDLVRKGNRMEVVHRLLQDSRELGYKPFLMTIVGLPTEEREEAEELYQFLSDYHD
TVTYQIADFIVERNSPIQLRPDDYGIHIDDDEQESFHHNLHFTRRAGYSDEEAQEVYRDI
LVRTMQRFKGAHEVDVEQERSRVAPDDSVYRLSLRAGSFALENYWVKHNNLPFEGLVPIG
YKVQQQWTDMTDKGTVFEIDPDIALGALAGAGSR
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Conserved Residues
Superfamily CAR This EFD conserves 3/3 Superfamily-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
316 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
321 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
324 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
Subgroup CAR This EFD conserves 3/3 Subgroup-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
316 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
321 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
324 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
Family CAR This EFD conserves 3/3 Family-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
316 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IEA
321 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IEA
324 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IEA

Catalyzed Reaction

gentamicin N-methyltransferase

+ 2 + + +
Gentamicin A2
81279		 S-adenosyl-L-methionine zwitterion
59789		 gentamycin A
28418		 S-adenosyl-L-homocysteine zwitterion
57856		 L-methionine zwitterion
57844		 5'-deoxyadenosine
17319

EC: | IntEnz: | Kegg: | BioCyc: | BRENDA: |

Curation History
Time Change Annotation Old Value New Value
May 14, 2014, 3:03 a.m. update curation agent holliday setDomainBoundaries.py
update domain end position 520 634
update domain start position 306 1
July 15, 2014, 6:05 a.m. update curation agent setDomainBoundaries.py holliday
Oct. 16, 2014, 6:25 a.m. update curation agent holliday setDomainBoundaries.py
July 13, 2015, 1:08 p.m. update subgroup B12-binding domain containing methyltransferase (Class B)
EC number assigned by UniProtKB accession ID.