Top Level Name

  ⌊ Superfamily (core) Radical SAM

    ⌊ Subgroup B12-binding domain containing

  ⌊ methyltransferase (Class B)

     ⌊ Family thioacetal methlytransferase (Swb9-like)

  ⌊ FunctionalDomain Methyltransferase Swb9 (ID 391500)

Superfamily Assignment Evidence Code(s) ISS
Family Assignment Evidence Code CFM PubMed:19514719
This entry was last updated onJune 10, 2017

References to Other Databases

Genbank

SpeciesGIAccessionProteome
Streptomyces sp. SNA15896 Taxon ID: 497689 283131230

Uniprot

Protein NameAccessionEC Number Identifier
n/a D2KTX8 D2KTX8_9ACTN (TrEMBL)

Sequence

Length of Enzyme (full-length): 679 | Length of Functional Domain: 679

1       10        20        30        40        50        60

MEESGRLSVHLVQQGVWDMPLESMPLASGYLKACVQTDERVRDRVDVSIRNFRGGVTLMR
MANELFAEGAPDVLAFSVLGWNIREFGALAETFKQLNPRGLVVFGGTHVANQAERTFRMF
DDVDVIVNGEGDLVLPDVLDAYLRGVERTALGDIAGITYRDAGGTVVTTPPRPRIQDLDV
IPSPFLTGAIPLLDDNDRFRYDVALMETNRGCPYKCSFCYWGGAVGQRVQSFSRARLRAE
LELFARLKVHTIVLCDANFGMLRADEEFVDDVIDLRERYGYPRAVEASWAKNKSKVFFNI
VRKMKKHGMRSSFTLALQTLDDTALHLMNRKNMKVNDWEDLAAWLNREGLDAYAELIWGA
PGETVESFLEGYDRLSRHVSRIAVYPLHLLPNTDYAEKKAEYGIVSVRGNVDDFEYILSH
NTMSFADNRRMKRFLFWARVMAENAVLRGTWVAIRDLSDLTQSQVLTKLDAFVHRVDDPA
AQPLRAWVAEAENSNTACGDAIKYLFGDPVARQLLKRWWTESVRPLLPPERGDLFDEVFR
YDLLTQPYYHHPDAAGRPERLDTETIRGERYLRRSGVTMRYDVPALLVALRTGQVTPAEL
TAREHTVDLFYRQGAENFIGSTNHEEVVHFVGLTRDEVEAAAAVRAAKRDAEDTDEDAQQ
VQGGRQGLSLTLSEHGGYS
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Conserved Residues
Superfamily CAR This EFD conserves 3/3 Superfamily-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
212 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
216 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
219 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
Subgroup CAR This EFD conserves 3/3 Subgroup-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
212 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
216 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
219 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
Family CAR This EFD conserves 3/3 Family-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
212 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IEA
216 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IEA
219 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IEA

Catalyzed Reaction

thioacetal methlytransferase

+ 6 + 3 + 3 + 3
Swb9 substrate 1
132470		 S-adenosyl-L-methionine zwitterion
59789		 UK 63052
87401		 S-adenosyl-L-homocysteine zwitterion
57856		 L-methionine zwitterion
57844		 5'-deoxyadenosine
17319

EC: | IntEnz: | Kegg: | BioCyc: | BRENDA: |

Curation History
Time Change Annotation Old Value New Value
May 14, 2014, 3:36 a.m. update curation agent holliday setDomainBoundaries.py
update domain end position 416 663
update domain start position 4 1
Oct. 15, 2016, 5:25 a.m. update domain end position 663 679
EC number assigned by UniProtKB accession ID.