Top Level Name

  ⌊ Superfamily (core) Enolase

    ⌊ Subgroup mandelate racemase

     ⌊ Family galactarate dehydratase 3

  ⌊ FunctionalDomain galactarate dehydratase 3 (ID 289)

Superfamily Assignment Evidence Code(s) ISS PubMed:24926996
Family Assignment Evidence Code IES PubMed:24926996
This entry was last updated onNov. 22, 2017

References to Other Databases

Genbank

SpeciesGIAccessionProteome
Agrobacterium tumefaciens str. C58 Taxon ID: 176299 15890786 NP_356458.1 (RefSeq) URP
Taxon ID: 1183400 499280568 WP_010973629.1 (RefSeq)
Agrobacterium tumefaciens Taxon ID: 358 796556354 KJX86060.1 (Genbank) URP
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Uniprot

Protein NameAccessionEC Number Identifier
n/a A0A083ZHY7 A0A083ZHY7_RHIRD (TrEMBL)
n/a A0A1S7QPY7 A0A1S7QPY7_9RHIZ (TrEMBL)
n/a A9CG74 A9CG74_AGRFC (TrEMBL)

Sequence

Length of Enzyme (full-length): 395 | Length of Functional Domain: 395

1       10        20        30        40        50        60

MKIDRMRVFVTRDKDRPRVIVALDTDDGMTGWGECYNHGPDLALPPVLDYLYNFLAGQDP
TRVEFLYNLMLQQNRFPPGALGLSAISALDHCLWDLAAKAVGVPVYKLLGGEVRNRIKVY
AGVYTAPDAPAARDEFDRLNEGWGFSAFKLSPWRVDIHAHRWGEVVRSSAEYFRTLRETV
RADYDIAFDAHAKIFEPSAARQLGNALAPYDPLFYEEPLRPENIEMYGDLKQGLNCTLAT
GESLYNRNEFLRLLQVKGADIIQPDICVVGGISEMRRIATLAEAHFVSIAPHNPMGPLAT
AVNVHFCAVQQNFRILEYRLPKGQVYAYGGNDLVKEEDVTRYVVDPYLPRDGYLELRPDR
PGWGVEMDEKAMQEDGYVHWQRRVPKRPDGSYAFA
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Conserved Residues
Superfamily CAR This EFD conserves 3/3 Superfamily-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
189 Asp (D) side chain metal binding ligand metal ligand -- binding ISS PubMed:8987982
216 Glu (E) side chain metal binding ligand metal ligand -- binding ISS PubMed:8987982
242 Glu (E) side chain metal binding ligand metal ligand -- binding ISS PubMed:8987982
Subgroup CAR This EFD conserves 5/5 Subgroup-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
189 Asp (D) side chain metal binding ligand metal ligand -- binding ICS
216 Glu (E) side chain metal binding ligand metal ligand -- binding ICS
242 Glu (E) side chain metal binding ligand metal ligand -- binding ICS
265 Asp (D) side chain controls pKa of catalytic His perturbates pKa -- spectator IDA
292 His (H) side chain proton abstraction (general base) proton relay -- reactant IDA
Family CAR This EFD conserves 7/7 Family-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
189 Asp (D) side chain metal binding ligand metal ligand -- binding ICS PubMed:24926996
191 His (H) side chain general acid proton relay -- reactant IME PubMed:24926996
216 Glu (E) side chain metal binding ligand metal ligand -- binding ICS PubMed:24926996
242 Glu (E) side chain metal binding ligand metal ligand -- binding ICS PubMed:24926996
265 Asp (D) side chain controls pKa of His that functions as general base activation -- spectator ICS PubMed:24926996
292 His (H) side chain general base proton relay -- reactant IME PubMed:24926996
317 Glu (E) side chain None -- ISS PubMed:24926996

Catalyzed Reaction

galactarate dehydratase 2/3

+
galactarate(2-)
16537		 3-deoxy-D-threo-hex-2-ulosarate(2-)
78267		 water
15377

EC: 4.2.1.- | IntEnz: 4.2.1.- | Kegg: 4.2.1.- | BioCyc: 4.2.1.- | BRENDA: 4.2.1.- |

Curation History
Time Change Annotation Old Value New Value
Nov. 3, 2014, 2:33 a.m. update curation agent sbrown setDomainBoundaries.py
July 9, 2015, 3:08 a.m. update curation agent setDomainBoundaries.py sbrown
update curation agent sbrown setDomainBoundaries.py
update domain end position 380 395
update name uncharacterized mandelate racemase subgroup sequence, enolase superfamily galactarate dehydratase 3
EC number assigned by UniProtKB accession ID.