Top Level Name

  ⌊ Superfamily (core) Enolase

    ⌊ Subgroup muconate cycloisomerase

  ⌊ muconate cycloisomerase (syn) like

  ⌊ FunctionalDomain 3-methylmuconate cycloisomerase (ID 13872)

Superfamily Assignment Evidence Code(s) ISS
This entry was last updated onNov. 22, 2017

References to Other Databases

Genbank

SpeciesGIAccessionProteome
Pseudomonas reinekei Taxon ID: 395598 111145985 AAY23362.2 (Genbank)

Uniprot

Protein NameAccessionEC Number Identifier
n/a Q4KPK2 Q4KPK2_9PSED (TrEMBL)

Sequence

Length of Enzyme (full-length): 373 | Length of Functional Domain: 373

1       10        20        30        40        50        60

MTQAKIESIETILVDLPTVRPHKLAMHTISQQTLVIIRLRCSDGIEGLGESTTVGGLSYA
NESPESIKTNIDVHLAPALIGMDASNFNAAMQRLDRIAKGNTFAKSGLESALLDAQGKRQ
GVAVSQLLGGRVRDSLEVGWVLASGNTARDIAEAEHFLDVRRHRIFKVKIGANPLEQDLK
HVVAIKQALGDRASVRVDVNQYWDEAQAIRGCQVLGDNGIDLIEQPISRINRAGQVRLNQ
RSLAPIMADESIESVEDAFSLAADGAASVFALKIAKNGGPRAVLRTAQIAESAGIALYGA
TMLEGAIGTLASAHAFLTLKKLAWGTELFGPLLLTEEIVNEAPVYRDFELHVPNTPGLGV
SLDEERLALFRRR
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Conserved Residues
Superfamily CAR This EFD conserves 3/3 Superfamily-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
198 Asp (D) side chain metal binding ligand metal ligand -- binding ISS PubMed:8987982
224 Glu (E) side chain metal binding ligand metal ligand -- binding ISS PubMed:8987982
249 Asp (D) side chain metal binding ligand metal ligand -- binding ISS PubMed:8987982
Subgroup CAR This EFD conserves 7/7 Subgroup-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
167 Lys (K) side chain Electrostatic stabilization of transition state electrostatic stabiliser -- spectator ISS PubMed:10336378
169 Lys (K) side chain abstracts alpha proton (base) proton relay -- reactant ICS PubMed:10336378
198 Asp (D) side chain metal binding ligand metal ligand -- binding ICS PubMed:9724714
224 Glu (E) side chain metal binding ligand metal ligand -- binding ICS PubMed:9724714
249 Asp (D) side chain metal binding ligand metal ligand -- binding ICS PubMed:9724714
273 Lys (K) side chain Stabilizes enolate anion intermediate electrostatic stabiliser -- spectator ICS PubMed:19220063
327 Glu (E) side chain general acid proton relay -- reactant ICS PubMed:7500361

Catalyzed Reactions

3-methylmuconate cycloisomerase

3-methyl-cis,cis-muconic acid
28958		 4-carboxymethyl-4-methylbut-2-en-1,4-olide
16766

EC: 5.5.1.- | IntEnz: 5.5.1.- | Kegg: 5.5.1.- | BioCyc: 5.5.1.- | BRENDA: 5.5.1.- |

3-chloromuconate cycloisomerase (non-dehalogenating)

+
(2-chloro-5-oxo-2,5-dihydro-2-furyl)acetate
58109		 3-chloro-cis,cis-muconate(2-)
17589		 hydron
15378

EC: 5.5.1.7 | IntEnz: 5.5.1.7 | Kegg: 5.5.1.7 | BioCyc: 5.5.1.7 | BRENDA: 5.5.1.7

muconate cycloisomerase

+
cis,cis-muconate
32379		 hydron
15378		 5-oxo-2,5-dihydro-2-furylacetate
58372

EC: 5.5.1.1 | IntEnz: 5.5.1.1 | Kegg: 5.5.1.1 | BioCyc: 5.5.1.1 | BRENDA: 5.5.1.1

Curation History
Time Change Annotation Old Value New Value
Nov. 3, 2014, 2:35 a.m. update curation agent updateSFLD2.py setDomainBoundaries.py
Oct. 15, 2015, 3:28 a.m. update curation agent setDomainBoundaries.py sbrown
remove family assignment evidence code IEA -
remove family muconate cycloisomerase (syn) -
update name muconate cycloisomerase (syn) 3-methylmuconate cycloisomerase
update subgroup muconate cycloisomerase muconate cycloisomerase (syn) like
update superfamily assignment evidence code IEA ISS
Feb. 10, 2017, 3:06 a.m. update curation agent sbrown setDomainBoundaries.py
EC number assigned by UniProtKB accession ID.