Top Level Name

  ⌊ Superfamily (core) Enolase

    ⌊ Subgroup mandelate racemase

     ⌊ Family xylonate dehydratase 1

  ⌊ FunctionalDomain xylonate dehydratase 1 (ID 50)

Superfamily Assignment Evidence Code(s) ISS
Family Assignment Evidence Code IES PubMed:20736170
This entry was last updated onNov. 21, 2017

References to Other Databases

Genbank

SpeciesGIAccessionProteome
Sulfolobus solfataricus Taxon ID: 2287 497674398 WP_009988582.1 (RefSeq) URP
Sulfolobus solfataricus Taxon ID: 2287 800906085 AKA78266.1 (Genbank) URP
Sulfolobus solfataricus Taxon ID: 2287 800903391 AKA75573.1 (Genbank) URP
Show All

Uniprot

Protein NameAccessionEC Number Identifier
n/a A0A0E3JWC5 A0A0E3JWC5_SULSF (TrEMBL)
n/a D0KNY8 D0KNY8_SULS9 (TrEMBL)
n/a Q97VG1 Q97VG1_SULSO (TrEMBL)

Sequence

Length of Enzyme (full-length): 393 | Length of Functional Domain: 393

1       10        20        30        40        50        60

MTKISEIEAYILGKEVTSAQWASLMVLVRVTTNDGRVGWGETVSALRAEAVANFVKKINT
VLKGNDVFNVEKNRLEWYKHDFNMTISLESTTAYSAVDIASWDIIGKELGAPLYKLLGGK
TRDKVLVYANGWYQNCVKPEDFAEKAKEIVKMGYKALKFDPFGPYFNDISKKGLDIAEER
VKAVREAVGDNVDILIEHHGRFNANSAIMIAKRLEKYNPLFMEEPIHPEDVEGLRKYRNN
TSLRIALGERIINKQQALYFMKEGLVDFLQADLYRIGGVTETKKVVGIAETFDVQMAFHN
AQGPILNAVTLQFDAFIPNFLIQESFYDWFPSWKRELIYNGTPIDNGYAIIPERPGLGVE
VNEKMLDSLKVKGEEYFNPEEPVWVVKGTWRDY
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Conserved Residues
Superfamily CAR This EFD conserves 3/3 Superfamily-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
197 Glu (E) side chain metal binding ligand metal ligand -- binding ISS PubMed:8987982
223 Glu (E) side chain metal binding ligand metal ligand -- binding ISS PubMed:8987982
249 Glu (E) side chain metal binding ligand metal ligand -- binding ISS PubMed:8987982
Subgroup CAR This EFD conserves 5/5 Subgroup-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
197 Glu (E) side chain metal binding ligand metal ligand -- binding ICS
223 Glu (E) side chain metal binding ligand metal ligand -- binding ICS
249 Glu (E) side chain metal binding ligand metal ligand -- binding ICS
272 Asp (D) side chain controls pKa of catalytic His perturbates pKa -- spectator IDA
299 His (H) side chain proton abstraction (general base) proton relay -- reactant IDA
Family CAR This EFD conserves 7/7 Family-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
197 Glu (E) side chain Metal binding ligand metal ligand -- binding ISS
199 His (H) side chain general acid proton relay -- reactant ISS
223 Glu (E) side chain Metal binding ligand metal ligand -- binding ISS
249 Glu (E) side chain Metal binding ligand metal ligand -- binding ISS
272 Asp (D) side chain Controls pKa of His base activation -- spectator ISS
299 His (H) side chain proton abstraction (general base) proton relay -- reactant ISS
324 Glu (E) side chain stabilizes transition state electrostatic stabiliser -- spectator ISS

Catalyzed Reaction

xylonate dehydratase

+
D-xylonate
17746		 2-dehydro-3-deoxy-D-arabinonic acid
1060		 water
15377

EC: 4.2.1.82 | IntEnz: 4.2.1.82 | Kegg: 4.2.1.82 | BioCyc: 4.2.1.82 | BRENDA: 4.2.1.82

Curation History
Time Change Annotation Old Value New Value
Nov. 3, 2014, 2:33 a.m. update curation agent sbrown setDomainBoundaries.py
July 9, 2015, 3:07 a.m. update curation agent setDomainBoundaries.py sbrown
update curation agent sbrown setDomainBoundaries.py
update family assignment evidence code ISS IES
update family gluconate dehydratase xylonate dehydratase 1
update name gluconate dehydratase xylonate dehydratase 1
EC number assigned by UniProtKB accession ID.