Top Level Name

  ⌊ Superfamily (core) Haloacid Dehalogenase

  ⌊ FunctionalDomain uncharacterized haloacid dehalogenase superfamily sequence (ID 477037)

Superfamily Assignment Evidence Code(s) IEA
This entry was last updated onFeb. 10, 2017

References to Other Databases

Genbank

SpeciesGIAccessionProteome
Mus musculus Taxon ID: 10090 256665234 NP_001157835.1 (RefSeq) PRP URP

Uniprot

Protein NameAccessionEC Number Identifier
Cytosolic purine 5'-nucleotidase Q3V1L4 3.1.3.5 5NTC_MOUSE (Swiss-Prot)

Sequence

Length of Enzyme (full-length): 586 | Length of Functional Domain: 586

1       10        20        30        40        50        60

MVPGSGPGARLRRDLARTRRIHPTNKMTTSWSDRLQNAADVPANMDKHALKKYRREAYHR
VFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDST
FPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRDDTERFYILN
TLFNLPETYLLACLVDFFTNCPRYTSCDTGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSL
KEKTVENLEKYVVKDGKLPLLLSRMKEVGKVFLATNSDYKYTDKIMTYLFDFPHGPKPGS
SHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDT
ICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQS
LDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQV
MRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESPLATRNRTSVDF
KDTDYKRHQLTRSISEIKPPNLFPLAPQEITHCHDEDDDEEEEEEE
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Structures

PDB ID Title Molecule Name Number of
EFDs		 Resolution (Å) Mutant? Het group Links
5CR7 Human Cytosolic 5'-Nucleotidase Ii In Complex With N-(9H-Purin-6-Yl)-3-(3-Pyrrol-1-Ylphenyl)Benzamide Cytosolic Purine 5'-Nucleotidase 9 2.9 ~{N}-(7~{H}-Purin-6-Yl)-3-(3-Pyrrol-1-Ylphenyl) Benzamide
(4 more ⇓) 		CSA • PDB • PDBSum
2JC9 Crystal Structure Of Human Cytosolic 5'-Nucleotidase Ii In Complex With Adenosine Cytosolic Purine 5'-Nucleotidase 19 1.5 Magnesium Ion
(3 more ⇓) 		CSA • PDB • PDBSum
2J2C Crystal Structure Of Human Cytosolic 5'-Nucleotidase Ii (Nt5C2, Cn-Ii) Cytosolic Purine 5'-Nucleotidase 19 2.2 Sulfate Ion
(2 more ⇓) 		CSA • PDB • PDBSum
4H4B Human Cytosolic 5'-Nucleotidase Ii In Complex With Anthraquinone-2,6-Disulfonic Acid Cytosolic Purine 5'-Nucleotidase 19 2.9 9,10-Dioxo-9,10-Dihydroanthracene-2,6-Disulfonic Acid
(3 more ⇓) 		CSA • PDB • PDBSum
5CQZ Human Cytosolic 5'-Nucleotidase Ii In Complex With 3-(3-Imidazol-1-Ylphenyl)-N-(9H-Purin-6-Yl)Benzamide Cytosolic Purine 5'-Nucleotidase 9 2.9 Glycerol
(3 more ⇓) 		CSA • PDB • PDBSum
2XJD Crystal Structure Of The D52N Variant Of Cytosolic 5'-Nucleotidase Ii In Complex With Inorganic Phosphate And Deoxyadenosine Triphosphate Cytosolic Purine 5'-Nucleotidase 17 2.0 Yes Adenosine-5'-Triphosphate
(3 more ⇓) 		CSA • PDB • PDBSum
2XJB Crystal Structure Of The D52N Variant Of Cytosolic 5'-Nucleotidase Ii In Complex With Deoxyguanosine Monophosphate And Deoxyadenosine Triphosphate Cytosolic Purine 5'-Nucleotidase 17 2.3 Yes Glycerol
(3 more ⇓) 		CSA • PDB • PDBSum
2XJC Crystal Structure Of The D52N Variant Of Cytosolic 5'-Nucleotidase Ii In Complex With Guanosine Monophosphate And Diadenosine Tetraphosphate Cytosolic Purine 5'-Nucleotidase 17 2.0 Yes Guanosine-5'-Monophosphate
(3 more ⇓) 		CSA • PDB • PDBSum
2XCW Crystal Structure Of The D52N Variant Of Cytosolic 5'-Nucleotidase Ii In Complex With Inosine Monophosphate And Atp Cytosolic Purine 5'-Nucleotidase 17 1.9 Yes Adenosine-5'-Triphosphate
(3 more ⇓) 		CSA • PDB • PDBSum
2XCV Crystal Structure Of The D52N Variant Of Cytosolic 5'-Nucleotidase Ii In Complex With Inosine Monophosphate And 2,3-Bisphosphoglycerate Cytosolic Purine 5'-Nucleotidase 17 2.3 Yes (2R)-2,3-Diphosphoglyceric Acid
(3 more ⇓) 		CSA • PDB • PDBSum
2XCX Crystal Structure Of The Apoform Of The D52N Variant Of Cytosolic 5'-Nucleotidase Ii Cytosolic Purine 5'-Nucleotidase 17 2.3 Yes Glycerol CSA • PDB • PDBSum
2XJF Crystal Structure Of The D52N Variant Of Cytosolic 5'-Nucleotidase Ii With A Covalently Modified Asn52 Cytosolic Purine 5'-Nucleotidase 17 2.1 Yes O-Carboxy-4-Imino-L-Homoserine
(2 more ⇓) 		CSA • PDB • PDBSum
2JCM Crystal Structure Of Human Cytosolic 5'-Nucleotidase Ii In Complex With Beryllium Trifluoride Cytosolic Purine 5'-Nucleotidase 17 2.15 Aspartate Beryllium Trifluoride
(3 more ⇓) 		CSA • PDB • PDBSum
2XJE Crystal Structure Of The D52N Variant Of Cytosolic 5'-Nucleotidase Ii In Complex With Uridine 5'-Monophosphate And Adenosine Triphosphate Cytosolic Purine 5'-Nucleotidase 17 2.3 Yes Uridine-5'-Monophosphate
(3 more ⇓) 		CSA • PDB • PDBSum
5K7Y Crystal Structure Of Enzyme In Purine Metabolism Cytosolic Purine 5'-Nucleotidase 8 1.79 Yes Glycerol CSA • PDB • PDBSum
5L50 Crystal Structure Of Enzyme In Purine Metabolism Cytosolic Purine 5'-Nucleotidase 8 1.64 Yes Glycerol CSA • PDB • PDBSum
5L4Z Crystal Structure Of Enzyme In Purine Metabolism Cytosolic Purine 5'-Nucleotidase 8 1.84 Yes Glycerol CSA • PDB • PDBSum
Percent identity and alignment length from the BLAST match of the Functional Domain sequence to the PDB sequence.
"Yes" indicates that a GI associated with this Functional Domain was mapped to the PDB ID via UniProtKB.

Curation History
Time Change Annotation Old Value New Value
April 30, 2014, 6:58 a.m. update curation agent sbrown setDomainBoundaries.py
update domain end position 289 586
update domain start position 229 1
EC number assigned by UniProtKB accession ID.