Top Level Name

  ⌊ Superfamily (core) Radical SAM

    ⌊ Subgroup BATS domain containing

  ⌊ HydE/PylB-like

  ⌊ FunctionalDomain uncharacterized HydE/PylB-like sequence (ID 419201)

Superfamily Assignment Evidence Code(s) IEA
This entry was last updated onJune 10, 2017

References to Other Databases

Genbank

SpeciesGIAccessionProteome
Thermotoga neapolitana DSM 4359 Taxon ID: 309803 221572668 ACM23480.1 (Genbank) URP

Uniprot

Protein NameAccessionEC Number Identifier
n/a B9K947 B9K947_THENN (TrEMBL)

Sequence

Length of Enzyme (full-length): 307 | Length of Functional Domain: 307

1       10        20        30        40        50        60

MRKKYVGDEVHIRAIIEFSNVCRKNCLYCGLRRDNKNLKRYRMTPEEIIERAKLAVQFGA
KTIVLQSGEDPYYMPDVISDIVREIKKMGVAVTLSLGEWPREFYEKWKEAGADRYLLRHE
TANPVLHKKLRPDTSFENRVRCLLTLKELGYETGAGSMVGLPGQTIDDLVDDLLFLKKYD
FDMVGIGPFIPHPDTPLANEKKGDFILTLKMVALTRILLPDSNIPATTAMGTIVPGGREI
TLRCGANVIMPNWTPSPYRQLYQLYPGKICVFEKDTACIPCVMKMIEHLGRRPGRDWGSR
KRVFETV
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Conserved Residues
Superfamily CAR This EFD conserves 3/3 Superfamily-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
22 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
26 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
29 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
Subgroup CAR This EFD conserves 3/3 Subgroup-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
22 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IEA
26 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IEA
29 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IEA

Structures

PDB ID Title Molecule Name Number of
EFDs		 Resolution (Å) Mutant? Het group Links
4JY8 X-Ray Snapshots Of Possible Intermediates In The Time Course Of Synthesis And Degradation Of Protein-Bound Fe4S4 Clusters Fefe-Hydrogenase Maturase 10 2.9 Iron/Sulfur Cluster
(3 more ⇓) 		CSA • PDB • PDBSum
4JY9 X-Ray Snapshots Of Possible Intermediates In The Time Course Of Synthesis And Degradation Of Protein-Bound Fe4S4 Clusters Fefe-Hydrogenase Maturase 10 1.6 Iron/Sulfur Cluster
(4 more ⇓) 		CSA • PDB • PDBSum
5FF2 Hyde From T. Maritima In Complex With (2R,4R)-Tda [Fefe] Hydrogenase Maturase Subunit Hyde 7 1.47 Iron/Sulfur Cluster
(5 more ⇓) 		CSA • PDB • PDBSum
Percent identity and alignment length from the BLAST match of the Functional Domain sequence to the PDB sequence.
"Yes" indicates that a GI associated with this Functional Domain was mapped to the PDB ID via UniProtKB.

Curation History
Time Change Annotation Old Value New Value
May 14, 2014, 3:08 a.m. update curation agent holliday setDomainBoundaries.py
update domain end position 252 307
Sept. 15, 2014, 5:17 a.m. update curation agent setDomainBoundaries.py holliday
update name uncharacterized Radical SAM superfamily sequence uncharacterized HydE protein
update subgroup - BATS domain containing
update superfamily assignment evidence code ISS IEA
Oct. 16, 2014, 6:46 a.m. update curation agent holliday setDomainBoundaries.py
May 11, 2015, 1:49 p.m. update curation agent setDomainBoundaries.py holliday
update curation agent holliday setDomainBoundaries.py
remove family assignment evidence code IEA -
remove family [FeFe] hydrogenase maturase (HydE-like) -
Dec. 17, 2015, 5:52 a.m. update curation agent setDomainBoundaries.py holliday
update name uncharacterized HydE protein uncharacterized HydE/PylB-like sequence
update subgroup BATS domain containing HydE/PylB-like
Oct. 15, 2016, 6:30 a.m. update curation agent holliday setDomainBoundaries.py
EC number assigned by UniProtKB accession ID.