Top Level Name

  ⌊ Superfamily (core) Radical SAM

    ⌊ Subgroup F420, menaquinone cofactor biosynthesis

  ⌊ 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase

     ⌊ Family 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase (CofG-like)

  ⌊ FunctionalDomain FO synthase subunit 1 (ID 379891)

Superfamily Assignment Evidence Code(s) ISS PubMed:14593448
Family Assignment Evidence Code ISS
This entry was last updated onJune 10, 2017

References to Other Databases

Genbank

SpeciesGIAccessionProteome
Methanocaldococcus jannaschii DSM 2661 Taxon ID: 243232 224471796 PRP URP

Uniprot

Protein NameAccessionEC Number Identifier
FO synthase subunit 1 Q57888 2.5.1.77 COFG_METJA (Swiss-Prot)

Sequence

Length of Enzyme (full-length): 358 | Length of Functional Domain: 331

1       10        20        30        40        50        60

MISREEAINFLNSTSSKDILDKLAQINNTFKREYITYSKNVFIPLSKWCRNKCGYCIFRE
DKPSLMKPNEVKEILLKGDRLGCREALFTFGEHVDENKEIKEQLKSMGYDNILEYLYDLE
EWTLNNTSLLPHTNCGILNYDELKMLKDVNASMGLMLENASERLMNTIAHKHSPGKHPKL
RIEMIENAGKLKIPFTTGLLIGIGETNEEIVDSLFKIKEIHEKYGHIQEVIIQNFRAKKG
IPMENFKEPSPIKMLKVIILAKLILDDISIQIPPNLNRETGQLFLLAGVDDWGGVSPLTR
DYVNPEAEWPEIKELREWTEELGLKLKMRLPVYDKYISEEWLSEKVYNKIIEMGWLKE
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Conserved Residues
Superfamily CAR This EFD conserves 3/3 Superfamily-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
49 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
53 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
56 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
Subgroup CAR This EFD conserves 3/3 Subgroup-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
49 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
53 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
56 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
Family CAR This EFD conserves 3/3 Family-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
49 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IMP PubMed:25781338
53 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IMP PubMed:25781338
56 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IMP PubMed:25781338

Catalyzed Reaction

CofG

+ + +
5-amino-5-(4-hydroxybenzyl)-6-((2,3,4,5-tetrahydroxypentyl)amino)dihydropyrimidine-2,4-dione(1+)
85512		 S-adenosyl-L-methionine zwitterion
59789		 7,8-didemethyl-8-hydroxy-5-deazariboflavin(1-)
59904		 5'-deoxyadenosine
17319		 L-methionine zwitterion
57844

EC: 5.-.-.- | IntEnz: 5.-.-.- | Kegg: 5.-.-.- | BioCyc: 5.-.-.- | BRENDA: 5.-.-.- |

Curation History
Time Change Annotation Old Value New Value
May 14, 2014, 3:29 a.m. update curation agent holliday setDomainBoundaries.py
update domain start position 1 28
May 11, 2015, 11:30 a.m. update curation agent setDomainBoundaries.py holliday
update curation agent holliday setDomainBoundaries.py
update family assignment evidence code ISS IDA
Dec. 17, 2015, 4:59 a.m. update curation agent setDomainBoundaries.py holliday
Jan. 18, 2016, 4:51 a.m. update curation agent holliday setDomainBoundaries.py
Oct. 15, 2016, 5:07 a.m. update curation agent setDomainBoundaries.py holliday
update curation agent holliday setDomainBoundaries.py
update family assignment evidence code IDA ISS
update subgroup F420, menaquinone cofactor biosynthesis 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase
EC number assigned by UniProtKB accession ID.