Top Level Name

  ⌊ Superfamily (core) Isoprenoid Synthase Type I

    ⌊ Subgroup Terpene Cyclase Like 1 C Terminal Domain

  ⌊ Terpene Cyclase Like 1 C Terminal Domain - Enzymatic

  ⌊ FunctionalDomain Abietadiene Synthase, C Terminal Domain (ID 3729)

Superfamily Assignment Evidence Code(s) IEA
This entry was last updated onJune 24, 2017

Related Enzyme Functional Domain(s)

Abietadiene synthase, N Terminal Domain

References to Other Databases

Genbank

SpeciesGIAccessionProteome
n/a 389728650 AFK97049.1 (Genbank)
n/a 189861999 ACE36350.1 (Genbank)
n/a 77372606 ABA68837.1 (Genbank)
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Uniprot

Protein NameAccessionEC Number Identifier
Bifunctional abietadiene synthase, chloroplastic Q38710 TPSDV_ABIGR (Swiss-Prot)

Sequence

Length of Enzyme (full-length): 868 | Length of Functional Domain: 327

1       10        20        30        40        50        60

MAMPSSSLSSQIPTAAHHLTANAQSIPHFSTTLNAGSSASKRRSLYLRWGKGSNKIIACV
GEGGATSVPYQSAEKNDSLSSSTLVKREFPPGFWKDDLIDSLTSSHKVAASDEKRIETLI
SEIKNMFRCMGYGETNPSAYDTAWVARIPAVDGSDNPHFPETVEWILQNQLKDGSWGEGF
YFLAYDRILATLACIITLTLWRTGETQVQKGIEFFRTQAGKMEDEADSHRPSGFEIVFPA
MLKEAKILGLDLPYDLPFLKQIIEKREAKLKRIPTDVLYALPTTLLYSLEGLQEIVDWQK
IMKLQSKDGSFLSSPASTAAVFMRTGNKKCLDFLNFVLKKFGNHVPCHYPLDLFERLWAV
DTVERLGIDRHFKEEIKEALDYVYSHWDERGIGWARENPVPDIDDTAMGLRILRLHGYNV
SSDVLKTFRDENGEFFCFLGQTQRGVTDMLNVNRCSHVSFPGETIMEEAKLCTERYLRNA
LENVDAFDKWAFKKNIRGEVEYALKYPWHKSMPRLEARSYIENYGPDDVWLGKTVYMMPY
ISNEKYLELAKLDFNKVQSIHQTELQDLRRWWKSSGFTDLNFTRERVTEIYFSPASFIFE
PEFSKCREVYTKTSNFTVILDDLYDAHGSLDDLKLFTESVKRWDLSLVDQMPQQMKICFV
GFYNTFNDIAKEGRERQGRDVLGYIQNVWKVQLEAYTKEAEWSEAKYVPSFNEYIENASV
SIALGTVVLISALFTGEVLTDEVLSKIDRESRFLQLMGLTGRLVNDTKTYQAERGQGEVA
SAIQCYMKDHPKISEEEALQHVYSVMENALEELNREFVNNKIPDIYKRLVFETARIMQLF
YMQGDGLTLSHDMEIKEHVKNCLFQPVA
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Conserved Residues
Superfamily CAR This EFD conserves 3/3 Superfamily-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
621 Asp (D) side chain None --
625 Asp (D) side chain None --
765 Asn (N) side chain None --
Subgroup CAR This EFD conserves 8/8 Subgroup-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
584 Arg (R) side chain Hydrogen bonds to PPi electrostatic stabiliser -- spectator ICS PubMed:12432096
621 Asp (D) side chain Metal ligand metal ligand -- binding ICS PubMed:12432096
622 Asp (D) side chain None -- ISS PubMed:16895335
625 Asp (D) side chain Metal ligand metal ligand -- binding ICS PubMed:12432096
632 Asp (D) side chain Electrostatic interaction with R near N-terminus assists in active site closure electrostatic stabiliser -- spectator ICS PubMed:17372193
762 Arg (R) side chain Hydrogen bonds to PPi electrostatic stabiliser -- spectator ICS PubMed:12432096
765 Asn (N) side chain Metal ligand metal ligand -- binding ICS PubMed:12432096
766 Asp (D) side chain None -- ISS PubMed:16895335

Structures

PDB ID Title Molecule Name Number of
EFDs		 Resolution (Å) Mutant? Het group Links
3S9V Abietadiene Synthase From Abies Grandis Abietadiene Synthase, Chloroplastic 10 2.3 CSA • PDB • PDBSum
Percent identity and alignment length from the BLAST match of the Functional Domain sequence to the PDB sequence.
"Yes" indicates that a GI associated with this Functional Domain was mapped to the PDB ID via UniProtKB.

Curation History
Time Change Annotation Old Value New Value
April 30, 2014, 11:58 a.m. update curation agent holliday setDomainBoundaries.py
update domain start position 542 64
May 31, 2014, 11:31 a.m. update domain start position 64 542
EC number assigned by UniProtKB accession ID.