Top Level Name

  ⌊ Superfamily (core) Isoprenoid Synthase Type I

    ⌊ Subgroup Terpene Cyclase Like 1 C Terminal Domain

  ⌊ Terpene Cyclase Like 1 C Terminal Domain - Enzymatic

  ⌊ FunctionalDomain uncharacterized Terpene Cyclase Like 1 C-term subgroup sequence, Isoprenoid Synthase Type I superfamily (ID 310998)

Superfamily Assignment Evidence Code(s) IEA
This entry was last updated onJune 19, 2017

Related Enzyme Functional Domain(s)

uncharacterized Terpene Cyclase Like 1 N-term subgroup sequence, Isoprenoid Synthase Type II superfamily

References to Other Databases

Genbank

SpeciesGIAccessionProteome
n/a 757015722 AJN59766.1 (Genbank)
Abies balsamea Taxon ID: 90345 343174800 AEL99951.1 (Genbank)
Abies balsamea Taxon ID: 90345 544604142

Uniprot

Protein NameAccessionEC Number Identifier
Bifunctional isopimaradiene synthase, chloroplastic H8ZM71 4.2.3.44 ISO_ABIBA (Swiss-Prot)

Sequence

Length of Enzyme (full-length): 852 | Length of Functional Domain: 328

1       10        20        30        40        50        60

HHLTANTQSIPHFSTTLNAGSSARKRRSLYLRWGKGSNKIIACVGEGATSVPYQSAEKND
SLYSSTLVKREFPPGFWKDDLIDSLTSSHKVAASDEKRIETLISEIKNMFRCMGYGETNP
SAYDTAWVARIPALDGSDNPHFPETVEWILQNQLKDGSWGEGFYFLAYDRILATLACIIT
LTLWRTGETQVHKGIEFFRTQAGKMEDEADSHRPSGFEIVFPAMLKEAKILGLDLPYDLP
FLKQIIEKREAKLKRIPTDVLYALPTTLLYSLEGLQEIVDWQKIMKLQSKDGSFLSSPAS
TAAVFMRTGNKKCLDFLNFVLKKFGNHVPCHYPLDLFERLWAVDTVERLGIDRHFKEEIK
DALDYVYSHWDERGIGWARENPVPDIDDTAMGLRILRLHGYNVSSDVLKTFRDENGEFFC
FLGQTQRGVTDMLNVNRCSHVSFPGETIMEEAKLCTERYLRNALENVDAFDKWAFKKNIR
GEVEYALKYTWHKSMPRLEARSYIENYGPNDAWLGKTVYRMPYISNEKYLELAKLDFNKL
QSIHQTELQDLRRWWKSSGFSKLNFTRERVTEIYFSSASFMFEPEFSKCREVYTKASIFT
LIFDDLYDAHGSLDDLKLFSEAVKRWDLSLLERMPQEMKICFLGFYNTFNEIAEEVHKRQ
GRDMLGHIQNVWEILLAAYTKEAEWSKTKYVPSFDEYIENASVSITLGTIVLISTLFIGE
VLTDHVLSKINHGSRFLHLMGLTGRLVNDTKTYQAERGQGEEASAIQCYMKDHPEISEEE
ALNHVYNVMENALQELNKEFVNNKEVPPNCRRLVFNTARIMQLFYMQGDGLTLSHDMEIK
DHVKTCLFIPIA
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Conserved Residues
Superfamily CAR This EFD conserves 3/3 Superfamily-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
604 Asp (D) side chain None --
608 Asp (D) side chain None --
748 Asn (N) side chain None --
Subgroup CAR This EFD conserves 8/8 Subgroup-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
567 Arg (R) side chain Hydrogen bonds to PPi electrostatic stabiliser -- spectator ICS PubMed:12432096
604 Asp (D) side chain Metal ligand metal ligand -- binding ICS PubMed:12432096
605 Asp (D) side chain None -- ISS PubMed:16895335
608 Asp (D) side chain Metal ligand metal ligand -- binding ICS PubMed:12432096
615 Asp (D) side chain Electrostatic interaction with R near N-terminus assists in active site closure electrostatic stabiliser -- spectator ICS PubMed:17372193
745 Arg (R) side chain Hydrogen bonds to PPi electrostatic stabiliser -- spectator ICS PubMed:12432096
748 Asn (N) side chain Metal ligand metal ligand -- binding ICS PubMed:12432096
749 Asp (D) side chain None -- ISS PubMed:16895335

Curation History
Time Change Annotation Old Value New Value
April 30, 2014, 12:12 p.m. update curation agent sbrown setDomainBoundaries.py
update domain start position 525 55
May 31, 2014, 11:43 a.m. update domain start position 55 525
EC number assigned by UniProtKB accession ID.