Top Level Name

  ⌊ Superfamily (core) Isoprenoid Synthase Type I

    ⌊ Subgroup Terpene Cyclase Like 1 C Terminal Domain

  ⌊ Terpene Cyclase Like 1 C Terminal Domain - Enzymatic

  ⌊ FunctionalDomain uncharacterized Terpene Cyclase Like 1 C-term subgroup sequence, Isoprenoid Synthase Type I superfamily (ID 310541)

Superfamily Assignment Evidence Code(s) IEA
This entry was last updated onJune 19, 2017

Related Enzyme Functional Domain(s)

uncharacterized Terpene Cyclase Like 1 N-term subgroup sequence, Isoprenoid Synthase Type II superfamily

References to Other Databases

Genbank

SpeciesGIAccessionProteome
Abies grandis Taxon ID: 46611 371927472
Abies grandis Taxon ID: 46611 371927471
Abies grandis Taxon ID: 46611 371927470
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Uniprot

Protein NameAccessionEC Number Identifier
Bifunctional abietadiene synthase, chloroplastic Q38710 TPSDV_ABIGR (Swiss-Prot)

Sequence

Length of Enzyme (full-length): 785 | Length of Functional Domain: 327

1       10        20        30        40        50        60

MVKREFPPGFWKDDLIDSLTSSHKVAASDEKRIETLISEIKNMFRCMGYGETNPSAYDTA
WVARIPAVDGSDNPHFPETVEWILQNQLKDGSWGEGFYFLAYDRILATLACIITLTLWRT
GETQVQKGIEFFRTQAGKMEDEADSHRPSGFEIVFPAMLKEAKILGLDLPYDLPFLKQII
EKREAKLKRIPTDVLYALPTTLLYSLEGLQEIVDWQKIMKLQSKDGSFLSSPASTAAVFM
RTGNKKCLDFLNFVLKKFGNHVPCHYPLDLFERLWAVDTVERLGIDRHFKEEIKEALDYV
YSHWDERGIGWARENPVPDIDDTAMGLRILRLHGYNVSSDVLKTFRDENGEFFCFLGQTQ
RGVTDMLNVNRCSHVSFPGETIMEEAKLCTERYLRNALENVDAFDKWAFKKNIRGEVEYA
LKYPWHKSMPRLEARSYIENYGPDDVWLGKTVYMMPYISNEKYLELAKLDFNKVQSIHQT
ELQDLRRWWKSSGFTDLNFTRERVTEIYFSPASFIFEPEFSKCREVYTKTSNFTVILDDL
YDAHGSLDDLKLFTESVKRWDLSLVDQMPQQMKICFVGFYNTFNDIAKEGRERQGRDVLG
YIQNVWKVQLEAYTKEAEWSEAKYVPSFNEYIENASVSIALGTVVLISALFTGEVLTDEV
LSKIDRESRFLQLMGLTGRLVNDTKTYQAERGQGEVASAIQCYMKDHPKISEEEALQHVY
SVMENALEELNREFVNNKIPDIYKRLVFETARIMQLFYMQGDGLTLSHDMEIKEHVKNCL
FQPVA
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Conserved Residues
Superfamily CAR This EFD conserves 3/3 Superfamily-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
538 Asp (D) side chain None --
542 Asp (D) side chain None --
682 Asn (N) side chain None --
Subgroup CAR This EFD conserves 8/8 Subgroup-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
501 Arg (R) side chain Hydrogen bonds to PPi electrostatic stabiliser -- spectator ICS PubMed:12432096
538 Asp (D) side chain Metal ligand metal ligand -- binding ICS PubMed:12432096
539 Asp (D) side chain None -- ISS PubMed:16895335
542 Asp (D) side chain Metal ligand metal ligand -- binding ICS PubMed:12432096
549 Asp (D) side chain Electrostatic interaction with R near N-terminus assists in active site closure electrostatic stabiliser -- spectator ICS PubMed:17372193
679 Arg (R) side chain Hydrogen bonds to PPi electrostatic stabiliser -- spectator ICS PubMed:12432096
682 Asn (N) side chain Metal ligand metal ligand -- binding ICS PubMed:12432096
683 Asp (D) side chain None -- ISS PubMed:16895335

Structures

PDB ID Title Molecule Name Number of
EFDs		 Resolution (Å) Mutant? Het group Links
3S9V Abietadiene Synthase From Abies Grandis Abietadiene Synthase, Chloroplastic 10 2.3 CSA • PDB • PDBSum
Percent identity and alignment length from the BLAST match of the Functional Domain sequence to the PDB sequence.
"Yes" indicates that a GI associated with this Functional Domain was mapped to the PDB ID via UniProtKB.

Curation History
Time Change Annotation Old Value New Value
April 30, 2014, 12:10 p.m. update curation agent sbrown setDomainBoundaries.py
update domain start position 459 5
May 31, 2014, 11:42 a.m. update domain start position 5 459
EC number assigned by UniProtKB accession ID.