Top Level Name

  ⌊ Superfamily (core) Radical SAM

    ⌊ Subgroup SPASM/twitch domain containing

  ⌊ main SPASM domain-containing

  ⌊ dehydrogenase like

     ⌊ Family anaerobic Cys-type sulfatase-maturating enzyme

  ⌊ FunctionalDomain Anaerobic sulfatase-maturating enzyme (ID 280399)

Superfamily Assignment Evidence Code(s) ISS
Family Assignment Evidence Code CFM PubMed:18408004 PubMed:17335281
This entry was last updated onJune 10, 2017

References to Other Databases

Genbank

SpeciesGIAccessionProteome
Clostridium perfringens Taxon ID: 1502 499909546 WP_011590280.1 (RefSeq) URP
Clostridium perfringens ATCC 13124 Taxon ID: 195103 110674675 ABG83662.1 (Genbank) URP
Clostridium perfringens ATCC 13124 Taxon ID: 195103 491668774 URP
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Uniprot

Protein NameAccessionEC Number Identifier
Anaerobic sulfatase-maturating enzyme Q0TTH1 1.8.98.- ANSME_CLOP1 (Swiss-Prot)

Sequence

Length of Enzyme (full-length): 370 | Length of Functional Domain: 370

1       10        20        30        40        50        60

MPPLSLLIKPASSGCNLKCTYCFYHSLSDNRNVKSYGIMRDEVLESMVKRVLNEANGHCS
FAFQGGEPTLAGLEFFEKLMELQRKHNYKNLKIYNSLQTNGTLIDESWAKFLSENKFLVG
LSMDGPKEIHNLNRKDCCGLDTFSKVERAAELFKKYKVEFNILCVVTSNTARHVNKVYKY
FKEKDFKFLQFINCLDPLYEEKGKYNYSLKPKDYTKFLKNLFDFWYEDFLNGNRVSIRYF
DGLLETILLGKSSSCGMNGTCTCQFVVESDGSVYPCDFYVLDKWRLGNIQDMTMKELFET
NKNHEFIKLSFKVHEECKKCKWFRLCKGGCRRCRDSKEDSALELNYYCQSYKEFFEYAFP
RLINVANNIK
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Conserved Residues
Superfamily CAR This EFD conserves 3/3 Superfamily-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
15 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
19 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
22 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
Subgroup CAR This EFD conserves 6/6 Subgroup-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
15 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
19 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
22 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
320 Cys (C) side chain Binds [4Fe-4S] cluster cofactor binding -- binding ISS
326 Cys (C) side chain Binds [4Fe-4S] cluster cofactor binding -- binding ISS
348 Cys (C) side chain Binds [4Fe-4S] cluster cofactor binding -- binding ISS
Family CAR This EFD conserves 10/10 Family-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
15 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
19 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
22 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
255 Cys (C) side chain Binds [4Fe-4S] cluster cofactor binding -- binding ISS
261 Cys (C) side chain Binds [4Fe-4S] cluster cofactor binding -- binding ISS
276 Cys (C) side chain Binds [4Fe-4S] cluster cofactor binding -- binding ISS
317 Cys (C) side chain Binds [4Fe-4S] cluster cofactor binding -- binding ISS
320 Cys (C) side chain Binds [4Fe-4S] cluster cofactor binding -- binding ISS
326 Cys (C) side chain Binds [4Fe-4S] cluster cofactor binding -- binding ISS
330 Cys (C) side chain Binds [4Fe-4S] cluster cofactor binding -- binding ISS

Catalyzed Reaction

Cys-type sulfatase maturase

+ + + + +
cysteine residue
32460		 water
15377		 S-adenosyl-L-methionine zwitterion
59789		 3-oxoalanine residue
85621		 hydrogen sulfide
16136		 5'-deoxyadenosine
17319		 L-methionine zwitterion
57844

EC: 1.1.99.- | IntEnz: 1.1.99.- | Kegg: 1.1.99.- | BioCyc: 1.1.99.- | BRENDA: 1.1.99.- |

Structures

PDB ID Title Molecule Name Number of
EFDs		 Resolution (Å) Mutant? Het group Links
4K36 His6 Tagged Ansmecpe With Bound Adomet Anaerobic Sulfatase-Maturating Enzyme 10 1.62 Iron/Sulfur Cluster
(2 more ⇓) 		CSA • PDB • PDBSum
4K39 Native Ansmecpe With Bound Adomet And Cp18Cys Peptide Anaerobic Sulfatase-Maturating Enzyme • Cp18Cys Peptide 10 1.78 Iron/Sulfur Cluster
(3 more ⇓) 		CSA • PDB • PDBSum
4K38 Native Ansmecpe With Bound Adomet And Kp18Cys Peptide Anaerobic Sulfatase-Maturating Enzyme • Kp18Cys Peptide 10 1.83 Iron/Sulfur Cluster
(3 more ⇓) 		CSA • PDB • PDBSum
4K37 Native Ansmecpe With Bound Adomet Anaerobic Sulfatase-Maturating Enzyme 10 1.62 Iron/Sulfur Cluster
(3 more ⇓) 		CSA • PDB • PDBSum
Percent identity and alignment length from the BLAST match of the Functional Domain sequence to the PDB sequence.
"Yes" indicates that a GI associated with this Functional Domain was mapped to the PDB ID via UniProtKB.

Curation History
Time Change Annotation Old Value New Value
Oct. 16, 2014, 7:37 a.m. update curation agent holliday setDomainBoundaries.py
EC number assigned by UniProtKB accession ID.