Top Level Name

  ⌊ Superfamily (core) Radical SAM

    ⌊ Subgroup F420, menaquinone cofactor biosynthesis

  ⌊ 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase

     ⌊ Family ((2,3,4,5-tetrahydroxypentyl)amino)dihydropyrimidine-2,4-dione synthase (CofH-like)

  ⌊ FunctionalDomain FO synthase subunit 2 (ID 280150)

Superfamily Assignment Evidence Code(s) ISS PubMed:14593448 PubMed:25781338
Family Assignment Evidence Code CFM PubMed:14593448 PubMed:25781338
This entry was last updated onJune 10, 2017

References to Other Databases

Genbank

SpeciesGIAccessionProteome
Methanocaldococcus jannaschii DSM 2661 Taxon ID: 243232 15669622 NP_248435.1 (RefSeq) PRP URP
Methanocaldococcus jannaschii Taxon ID: 2190 499173362 WP_010870949.1 (RefSeq)
Methanocaldococcus jannaschii DSM 2661 Taxon ID: 243232 1500312 AAB99441.1 (Genbank) PRP URP
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Uniprot

Protein NameAccessionEC Number Identifier
FO synthase subunit 2 Q58826 2.5.1.77 COFH_METJA (Swiss-Prot)

Sequence

Length of Enzyme (full-length): 359 | Length of Functional Domain: 358

1       10        20        30        40        50        60

MDPNKFREKEISKKEALELFEDNEIIFELFKFADSLRREEVGDIVTYVVNRNINFTNICV
GNCRFCAFRANENDKHAYFLDIDEIAKRAVEAKKFGCTEVCIQGGLHPKIDTYYQAEILK
AVHEATKPYGDIHIHAFSPMEVYFGAENAGLDIKEALKILKENGLNSMPGTAAEILDDDI
RAELCPNKIKTKEWIYIIKEAHKLGIPTTATMMYGHIEEYKHWVNHLFIIKEIQEETNGF
TEFVPLSFMHKYAPIYKEGKAKAGATGIEDLKVFAVSRIIFKGLIKNIQASWVKLGKKMV
QVALRCGANDVGGTLIEESISRSAGAEHGVYMSVEEIRDMIKRVGLIPKERTTLYKILE
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Conserved Residues
Superfamily CAR This EFD conserves 3/3 Superfamily-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
59 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
63 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
66 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
Subgroup CAR This EFD conserves 3/3 Subgroup-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
59 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
63 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
66 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding ISS
Family CAR This EFD conserves 3/3 Family-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
59 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IMP PubMed:25781338
63 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IMP PubMed:25781338
66 Cys (C) side chain Binds [4Fe-4S]-AdoMet cluster cofactor binding -- binding IMP PubMed:25781338

Catalyzed Reaction

CofH

+ + + + +
tyrosine
18186		 S-adenosyl-L-methionine zwitterion
59789		 5-amino-6-(D-ribitylamino)uracil
15934		 dehydroglycine
53647		 5'-deoxyadenosine
17319		 L-methionine zwitterion
57844		 5-amino-5-(4-hydroxybenzyl)-6-((2,3,4,5-tetrahydroxypentyl)amino)dihydropyrimidine-2,4-dione(1+)
85512

EC: 2.5.1.- | IntEnz: 2.5.1.- | Kegg: 2.5.1.- | BioCyc: 2.5.1.- | BRENDA: 2.5.1.- |

Curation History
Time Change Annotation Old Value New Value
May 14, 2014, 3 a.m. update curation agent holliday setDomainBoundaries.py
update domain start position 1 2
July 15, 2014, 3:58 a.m. update curation agent setDomainBoundaries.py holliday
update family assignment evidence code CFM,ISS CFM
Oct. 16, 2014, 3:53 a.m. update curation agent holliday setDomainBoundaries.py
May 11, 2015, 8:44 a.m. update curation agent setDomainBoundaries.py holliday
update curation agent holliday setDomainBoundaries.py
Dec. 17, 2015, 4:08 a.m. update curation agent setDomainBoundaries.py holliday
Jan. 18, 2016, 3:56 a.m. update curation agent holliday setDomainBoundaries.py
Oct. 15, 2016, 3:52 a.m. update curation agent setDomainBoundaries.py holliday
update curation agent holliday setDomainBoundaries.py
update subgroup F420, menaquinone cofactor biosynthesis 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase
EC number assigned by UniProtKB accession ID.