Top Level Name

  ⌊ Superfamily (core) Isoprenoid Synthase Type II

    ⌊ Subgroup Prenyltransferase Like 2

     ⌊ Family   private

  ⌊ FunctionalDomain Lanosterol (ID 148058)

Superfamily Assignment Evidence Code(s) IEA
Family Assignment Evidence Code IEA
This entry was last updated onJune 10, 2017

References to Other Databases

Genbank

SpeciesGIAccessionProteome
Homo sapiens Taxon ID: 9606 119629705 EAX09300.1 (Genbank) PRP URP

Sequence

Length of Enzyme (full-length): 733 | Length of Functional Domain: 728

1       10        20        30        40        50        60

MRGGPRCLRRRGGPYKTEPATDLGRWRLNCERGRQTWTYLQDERAGREQTGLEAYALGLD
TKNYFKDLPKAHTAFEGALNGMTFYVGLQAEDGHWTGDYGGPLFLLPGLLITCHVARIPL
PAGYREEIVRYLRSVQLPDGGWGLHIEDKSTVFGTALNYVSLRILGVGPDDPDLVRARNI
LHKKGGAVAIPSWGKFWLAVLNVYSWEGLNTLFPEMWLFPDWAPAHPSTLWCHCRQVYLP
MSYCYAVRLSAAEDPLVQSLRQELYVEDFASIDWLAQRNNVAPDELYTPHSWLLRVVYAL
LNLYEHHHSAHLRQRAVQKLYEHIVADDRFTKSISIGPISKTINMLVRWYVDGPASTAFQ
EHVSRIPDYLWMGLDGMKMQGTNGSQIWDTAFAIQALLEAGGHHRPEFSSCLQKAHEFLR
LSQVPDNPPDYQKYYRQMRKGGFSFSTLDCGWIVSDCTAEALKAVLLLQEKCPHVTEHIP
RERLCDAVAVLLNMRNPDGGFATYETKRGGHLLELLNPSEVFGDIMIDYTYVECTSAVMQ
ALKYFHKRFPEHRAAEIRETLTQGLEFCRRQQRADGSWEGSWGVCFTYGTWFGLEAFACM
GQTYRDGTACAEVSRACDFLLSRQMADGGWGEDFESCEERRYLQSAQSQIHNTCWAMMGL
MAVRHPDIEAQERGVRCLLEKQLPNGDWPQENIAGVFNKSCAISYTSYRNIFPIWALGRF
SQLYPERALAGHP
This shows the full-length sequence of the enzyme. The region of the functional domain is highlighted in black letters, while the residual residues are shown in grey. The Superfamily domain, when present, is shown using underlining. In many cases the functional domain is the full-length sequence.
Conserved catalytic residues (as determined by automated alignment to family, subgroup, or superfamily HMMs) are shown with teal highlighting . Conserved catalytic residues which do not matched the Conserved Alignment Residue are shown with maroon highlighting . Information regarding their function can be found in the Conserved Residues section below.
FASTA formatted full-length sequence.
BLAST this sequence against SFLD.
Scan SFLD-HMMs with this sequence.

Conserved Residues

Subgroup CAR This EFD conserves 4/4 Subgroup-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
388 Trp (W) side chain stabilizes intermediate electrostatic stabiliser -- spectator ICS PubMed:9931258
445 Phe (F) side chain stabilizes intermediate electrostatic stabiliser -- spectator ICS PubMed:9931258
456 Asp (D) side chain initiates catalyzed reaction via protonation of substrate activation -- spectator ICS PubMed:9931258
582 Trp (W) side chain stabilizes intermediate electrostatic stabiliser -- spectator ICS PubMed:9931258
Family CAR This EFD conserves 5/5 Family-specific Conserved Alignment Residues.
Position Amino Acid Location Function Role Evidence Code Reference
388 Trp (W) side chain Stabilizes positive charge on C4 of intermediate electrostatic stabiliser -- spectator ISS
445 Phe (F) side chain Stabilizes positive charge on C8 of intermediate electrostatic stabiliser -- spectator ISS
456 Asp (D) side chain Initiates catalyzed reaction via protonation of substrate proton relay -- reactant ISS
582 Trp (W) side chain Stabilizes positive charge on C10 of intermediate electrostatic stabiliser -- spectator ISS
697 Phe (F) side chain Stabilizes positive charge on C18 of intermediate electrostatic stabiliser -- spectator ISS

Structures

PDB ID Title Molecule Name Number of
EFDs		 Resolution (Å) Mutant? Het group Links
1W6J Structure Of Human Osc In Complex With Ro 48-8071 Lanosterol Synthase 32 2.2 B-Octylglucoside
(2 more ⇓) 		CSA • PDB • PDBSum
1W6K Structure Of Human Osc In Complex With Lanosterol Lanosterol Synthase 32 2.1 B-Octylglucoside • Lanosterol CSA • PDB • PDBSum
Percent identity and alignment length from the BLAST match of the Functional Domain sequence to the PDB sequence.
"Yes" indicates that a GI associated with this Functional Domain was mapped to the PDB ID via UniProtKB.

Curation History
Time Change Annotation Old Value New Value
May 2, 2014, 3 a.m. update curation agent updateSuperfamily.py setDomainBoundaries.py
update domain end position 733 730
update domain start position 7 3
EC number assigned by UniProtKB accession ID.