Reaction: [formate-C-acetyltransferase]-activating enzyme List of reactions
| Reaction name: | [formate-C-acetyltransferase]-activating enzyme | Reaction type: | cognate | ||
|---|---|---|---|---|---|
| EC number: | 1.97.1.4 | Kegg ID: | R04710 | Rhea ID: | |
| Directionality: | reversible | Curation status: | valid | Classification: | public |
| Description: | A single glycine residue in EC 2.3.1.54, formate C-acetyltransferase, is oxidized to the corresponding radical by transfer of H from its CH2 to S-adenosyl-L-methionine (AdoMet) with concomitant cleavage of the latter. | ||||
| Curator notes: | This reaction is missing a structure for the semi-quinone product, therefore will not chemically balance at this time. | ||||
Substrate
| ID Type | Compound ID | Name | Stoichiometry | Curator notes |
|---|---|---|---|---|
| chebi | 67040 | S-adenosyl-L-methioninate | 1 | |
| pdb | 2pfl | Pyruvate Formate-Lyase (inactive) | 1 | Specifically, the Gly residue that is to become an activated glycyl radical. |
| chebi | 62787 | 1,5-dihydroflavin | 1 |
Product
| ID Type | Compound ID | Name | Stoichiometry | Curator notes |
|---|---|---|---|---|
| chebi | 57844 | L-methionine zwitterion | 1 | |
| chebi | 17319 | 5'-deoxyadenosine | 1 | |
| chebi | 15817 | semiquinone | 1 | |
| pdb | 2pfl | Pyruvate Formate-Lyase (active) | 1 | The PFL protein is now in its activated glycyl form. |
Schemes
| # | Name |
|---|---|
| 121 | [pyruvate-formate lyase]-activating enzyme, radical SAM superfamily |
Reaction
[formate-C-acetyltransferase]-activating enzyme
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| S-adenosyl-L-methioninate 67040 |
Pyruvate Formate-Lyase (inactive) 2pfl |
1,5-dihydroflavin 62787 |
L-methionine zwitterion 57844 |
5'-deoxyadenosine 17319 |
semiquinone 15817 |
Pyruvate Formate-Lyase (active) 2pfl |
EC: 1.97.1.4 | IntEnz: 1.97.1.4 | Kegg: 1.97.1.4 | BioCyc: 1.97.1.4 | BRENDA: 1.97.1.4