Top Level Name

  ⌊ Superfamily (core) Radical SAM

    ⌊ Subgroup methylthiotransferase

     ⌊ Family threonylcarbamoyladenosine tRNA methylthiotransferase

Total 100% <100%
Functional domains 2975 2963 12
UniProtKB 4669 4660 9
GI 9329 9293 36
Structures 0
Reactions 1
Functional domains of this family were last updated on June 10, 2017
New functional domains were last added to this family on June 22, 2014

Catalyses the methylthiolation of N6-threonylcarbamoyladenosine (t6A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A) at position 37 in tRNAs that read codons beginning with adenine. Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine

Arragain S, Handelman SK, Forouhar F, Wei FY, Tomizawa K, Hunt JF, Douki T, Fontecave M, Mulliez E, Atta M

Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA

▸ Abstract

J Biol Chem. 2010;285(37):28425-28433 | PubMed ID: 20584901

Anton BP, Russell SP, Vertrees J, Kasif S, Raleigh EA, Limbach PA, Roberts RJ

Functional characterization of the YmcB and YqeV tRNA methylthiotransferases of Bacillus subtilis

▸ Abstract

Nucleic Acids Res. 2010;38(18):6195-6205 | PubMed ID: 20472640

No notes.

Static File Downloads

File Name Description Parameters Stats
repnet.fam295.th50.pE20.mek250K.xgmml Representative network: each node is a group of similar sequences node similarity threshold = 50
max edge count = 250K
min -log10 E = 20		 size = 11M
num_edges = 31013
num_nodes = 250
sfld_alignment_fam295.msa Annotated Sequence Alignment, Stockholm format 126 sequences
size: 74K

Total number of functional domains in this group.
Number of Functional Domains that have been manually or automatically been assigned to a family.
Number of Functional Domains that have not been assigned to a family.
Number of structures available from the PDB for members of this group.
Number of Functional Domains with 100% of Conserved Residues
Number of Functional Domains with less than 100% Conserved Residues

Catalyzed Reaction(s)

tRNA (N6-L-threonylcarbamoyladenosine37-C2)-methylthiotransferase

+ 2 + + + +
N(6)-L-threonylcarbamoyladenine 5'-monophosphate(2-) residue
74418		 S-adenosyl-L-methionine zwitterion
59789		 Sulfur donor
80867		 2-thio-N(6)-L-threonylcarbamoyladenine 5'-monophosphate(2-) residue
74419		 5'-deoxyadenosine
17319		 L-methionine zwitterion
57844		 S-adenosyl-L-homocysteine zwitterion
57856

EC: 2.8.4.5 | IntEnz: 2.8.4.5 | Kegg: 2.8.4.5 | BioCyc: 2.8.4.5 | BRENDA: 2.8.4.5