SFLD Evidence Codes

	CFM (Canonical Family Member)
		Context			Definition
		Family Assignment			Indicates that a protein/domain is one of the canonical members of a family.

	FSM (Founding Superfamily Member)
		Context			Definition
		Superfamily Assignment			Indicates that a protein/domain is one of the founding superfamily members used to create the definition of a superfamily.

	ICR (Inferred from Catalytic Residues)
		Context			Definition
		Reaction Assignment			Used when reaction assignment is based on the presence of homologs to catalytic residues shown in similar enzymes to be required for the reaction (via a direct assay).
		Partial Reaction Assignment			Used when partial reaction assignment is based on the presence of homologs to catalytic residues shown in similar enzymes to be required for the partial reaction (via a direct assay).

	ICS (Inferred from Crystal Structure)
		Context			Definition
		Catalytic Residue Assignment			Used when catalytic residue assignment is based on examination of a crystal structure (ex. Metal binding ligands).
		Partial Reaction Assignment			Used when a theorized partial reaction is based on evidence from an X-ray crystal structure.

	IDA (Inferred from Direct Assay)
		Context			Definition
		Reaction Assignment			Used when an enzyme is directly assayed for a reaction (for example, by monitoring the disappearance of reactant or appearance of product spectrophotometrically).
		Partial Reaction Assignment			Used when there is experimental evidence to support a partial reaction (for example, the intermediate has been trapped).
		Conserved Residue Assignment			Used when there is experimental evidence that suggests a particular amino acid is catalytically important (for example, site directed mutagenesis experiments).

	IEA (Inferred from Electronic Annotation)
		Context			Definition
		Reaction Assignment			Used when a reaction is assigned based on electronic annotation (for example, an NCBI definition line), and has not been reviewed for accuracy by a human curator. Also used when reaction assignment is based on overall sequence or structural similarity to an enzyme/domain with reaction already assigned and has not been reviewed for accuracy by a human curator.
		Partial Reaction Assignment			Used when partial reaction assignment is based on overall sequence or structural similarity to an enzyme/domain with partial reaction already assigned, and has not been reviewed for accuracy by a human curator.
		Conserved Residue Assignment			Used when conserved residue assignment is based on an alignment to a family/superfamily member with conserved residues already assigned and has not been reviewed for accuracy by a human curator.
		Superfamily Assignment			Used when superfamily assignment is based on overall sequence or structural similarity to existing superfamily members and has not been reviewed for accuracy by a human curator.
		Family Assignment			Used when family assignment is based on overall sequence or structural similarity to existing family members and has not been reviewed for accuracy by a human curator.

	IES (Inferred from Experiment and Sequence)
		Context			Definition
		Family Assignment			Used when family membership is assigned based on sequence/structural similarity to existing family members as well as a direct assay that shows that the enzyme/domain in question catalyzes the canonical family reaction.

	IGS (Inferred from Genome Context and Sequence)
		Context			Definition
		Family Assignment			Used when family membership is assigned based on sequence/structural similarity to existing family members, as well as genome context information (the genomic region containing the gene for the putative family member also contains genes for other enzymes in the same biological pathway that the family enzymes are a part of).
		Reaction Assignment			Used when reaction assignment is made based on sequence/structural similarity to other sequences known from experimental evidence to catalyze the reaction, as well as genome context information (the genomic region containing the gene for the enzyme to be assigned the reaction also contains genes for other enzymes in the same biological pathway that reaction is a part of).

	IME (Inferred from Mutagenesis Experiment)
		Context			Definition
		Partial Reaction Assignment			Used when there is mutagenesis data that supports a particular partial reaction.

	ISS (Inferred from Sequence or Structural Similarity)
		Context			Definition
		Reaction Assignment			Used when reaction assignment is based on overall sequence or structural similarity to an enzyme/domain with reaction already assigned, and has been reviewed for accuracy by a human curator.
		Partial Reaction Assignment			Used when partial reaction assignment is based on overall sequence or structural similarity to an enzyme/domain with partial reaction already assigned, and has been reviewed for accuracy by a human curator.
		Conserved Residue Assignment			Used when conserved residue assignment is based on an alignment to a family/superfamily member with conserved residues already assigned, and has been reviewed for accuracy by a human curator.
		Superfamily Assignment			Used when superfamily assignment is based on overall sequence or structural similarity to existing superfamily members that has been reviewed for accuracy by a human curator.
		Family Assignment			Used when family assignment is based on overall sequence or structural similarity to existing family members that has been reviewed for accuracy by a human curator.

	TPR (Theorized Partial Reaction)
		Context			Definition
		Partial Reaction Assignment			Used when a partial reaction is assigned based on theory rather than experimental evidence.