Top Level Name

  ⌊ Superfamily (extended) SPOUT Methyltransferase

Family known
Total 100% <100% Family unknown
Functional domains 2593 0 0 2593
UniProtKB 15859 0 0 15859
GI 28400 0 0 28400
Structures 5
Reactions 0
Functional domains of this superfamily were last updated on Jan. 14, 2017
New functional domains were last added to this superfamily on May 7, 2013

The SPOUT methyltransferases (MTases) are a large class of S-adenosyl-L-methionine-dependent enzymes that exhibit an unusual alpha/beta fold with a very deep topological knot. Historically, it has been the 4th unrelated type of AdoMet-dependent MTase structure determined by X-ray crystallography, hence named 'class IV'. Nonetheless, only a few SPOUT members have been characterized functionally. Thus far, all of them have been found to be involved in post-transcriptional RNA modification and introduce methylation of 2'-OH groups of ribose (in tRNA – Trm3, TrmH, or rRNA – RlmB), or the N-1 atom of guanosine 37 in tRNA (TrmD), or the N-3 atom of uridine 1498 in 16S rRNA (RsmE).

Tkaczuk KL, Dunin-Horkawicz S, Purta E, Bujnicki JM

Structural and evolutionary bioinformatics of the SPOUT superfamily of methyltransferases

▸ Abstract

BMC Bioinformatics 2007;8(73):None-None | PubMed ID: 17338813

Nonetheless, only a few SPOUT members have been characterized functionally. Thus far, all of them have been found to be involved in posttranscriptional RNA modification and introduce methylation of 2'-OH groups of ribose (in tRNA – Trm3 [8], TrmH [9], or rRNA – RlmB [3]), or the N-1 atom of guanosine 37 in tRNA (TrmD, [10,11]), or the N-3 atom of uridine 1498 in 16S rRNA (RsmE; [12]).

Sequence Similarity Networks

Download a Sequence Similarity Network of this superfamily (XGMML format ).

Network downloads are XGMML files that are readable by program such as Cytoscape. In these networks, nodes represent proteins and edges represent pairwise similarities better than a given edge-score cutoff. The edge score is either a bit score for full networks or mean E values for a Repnet. Additionally, these networks contain several attributes with data from the SFLD.

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Full sequence similarity network is not available for this superfamily due to its size.
Repnet: 50% ID (22-Apr-2016)

List of files included in the download. A detailed list of included node attributes, their definitions, and their uses [revised: 1/24/2014].

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Multiple Sequence Alignment

View the alignment of a representative set of sequences of this superfamily using

Multiple Sequence Alignment

Align one or multiple sequences to the alignment of a representative set of sequences of this superfamily.
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Downloads

Data Type All (#) Known (#) Unknown (#)
Full length FASTA (2593) (0) (2593)
Complete annotation (.tsv) (2593) (0) (2593)
Annotation suitable for Excel ® (.tsv) (2593) (0) (2593)
Clear form

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To identify your sequence later, please make sure to provide a header line starting with '>' for each sequence. Empty headers are allowed, but a header line is always required.
Cutoff Value
The least significant edge-score at which pairwise similarities are included in the network. A bit score for the full network, or a mean E value for the Repnet.
XGMML format
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Download the annotation of all sequences as shown in the table below as a ͟Tab ͟Separated ͟Value (TSV) file. This file can be imported into a spreadsheet application.
Full length FASTA
Full length sequences in FASTA format.
Functional Domain FASTA
Sequences of the Functional Domain in FASTA format.
Complete annotation
Download complete annotation of sequences sets of this superfamily as a ͟Tab ͟Separated ͟Value (TSV) file. This file has all data but cell size can exceed the maximum supported by spreadsheet programs (such as Microsoft Excel ®).
Spreadsheet ready annotation
Annotation of sequences sets of this superfamily in a ͟Tab ͟Separated ͟Value (TSV) file. This file can be imported into a spreadsheet application. Cells which exceed the allowed spreadsheet maximum (32.5K) are preceded by the word "Truncated" and clipped short.
Total number of functional domains in this group.
Number of Functional Domains that have been manually or automatically been assigned to a family.
Number of Functional Domains that have not been assigned to a family.
Number of structures available from the PDB for members of this group.
Number of Functional Domains with 100% of Conserved Residues
Number of Functional Domains with less than 100% Conserved Residues