Top Level Name

  ⌊ Superfamily (core) Enolase

Family known
Total 100% <100% Family unknown
Functional domains 48850 31676 260 16914
UniProtKB 90829 68914 283 21632
GI 189102 136765 815 51522
Structures 371
Reactions 23
Functional domains of this superfamily were last updated on June 28, 2017
New functional domains were last added to this superfamily on Sept. 13, 2017

Enzymes in the enolase superfamily are related by their ability to catalyze the abstraction of a proton alpha to a carboxylic acid to form an enolate anion intermediate. This conserved partial reaction is mediated by conserved residues in the active site, including three residues used to bind a divalent metal involved in stabilization of the common intermediate. Reactions catalyzed by enzymes in this superfamily include racemization, beta-elimination of OH- or NH3, and cycloisomerization. Enzymes in the superfamily have two structural domains--an N-terminal capping domain and a C-terminal TIM beta/alpha-barrel domain, both of which are required for function. In general, the catalytic residues can be found at ends of the beta strands in the C-terminal barrel domain, while the residues required for substrate specificity are contained in the N-terminal domain. Although the TIM beta/alpha-barrel fold is common to many other superfamilies, none of these superfamilies show a significant level of sequence or functional similarity to the enolase superfamily.

Babbitt, P.C., et al.

The enolase superfamily: a general strategy for enzyme-catalyzed abstraction of the alpha-protons of carboxylic acids

▸ Abstract

Biochemistry 1996;35(51):16489-16501 | PubMed ID: 8987982

EFI

abstraction of proton alpha to carboxylate | Evidence Code: IDA

Sequence Similarity Networks

Download a Sequence Similarity Network of this superfamily (XGMML format ).

Network downloads are XGMML files that are readable by program such as Cytoscape. In these networks, nodes represent proteins and edges represent pairwise similarities better than a given edge-score cutoff. The edge score is either a bit score for full networks or mean E values for a Repnet. Additionally, these networks contain several attributes with data from the SFLD.

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Maximum number of edges:
250K 500K 750K ALL
edge-score cutoff

Full sequence similarity network is not available for this superfamily due to its size.
Repnet: 50% ID (8-Mar-2017)

List of files included in the download. A detailed list of included node attributes, their definitions, and their uses [revised: 1/24/2014].

Reaction Network

Download Reaction Network of this superfamily (XGMML format ).

Reaction Network downloads are XGMML files that are readable by program such as Cytoscape. In these networks, nodes represent proteins and edges represent pairwise similarities better than a given bitscore-cutoff. Additionally, these networks contain several attributes with data from the SFLD.

Reaction Network: 20-Dec-2016

Molecular Networks

Download Molecular Networks of this superfamily (XGMML format ).

Molecular Network downloads are XGMML files that are readable by program such as Cytoscape. In these networks, nodes represent molecules in a reaction and edges represent pairwise similarities better than a given bitscore-cutoff. Additionally, these networks contain several attributes with data from the SFLD.

Molecular Network: Superfamily (23-Jan-2017) Molecular Network: Superfamily substrate (23-Jan-2017) Molecular Network: Superfamily product (23-Jan-2017)

Disclaimer
Although the download speed has improved please keep in mind that network files can be quite large. We are currently working on improving the network download and finding ways to make large networks manageable. Please see How to increase memory for Cytoscape.

Multiple Sequence Alignment

View the alignment of a representative set of sequences of this superfamily using

Multiple Sequence Alignment

Align one or multiple sequences to the alignment of a representative set of sequences of this superfamily.
Enter FASTA formatted sequence(s) : and view results using

Downloads

Data Type All (#) Known (#) Unknown (#)
Full length FASTA (48850) (31936) (16914)
Complete annotation (.tsv) (48850) (31936) (16914)
Annotation suitable for Excel ® (.tsv) (48850) (31936) (16914)
Clear form

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To identify your sequence later, please make sure to provide a header line starting with '>' for each sequence. Empty headers are allowed, but a header line is always required.
Cutoff Value
The least significant edge-score at which pairwise similarities are included in the network. A bit score for the full network, or a mean E value for the Repnet.
XGMML format
Open in Cytoscape via:
 Menu
 →Import
 →Network (multiple file types)
Download the annotation of all sequences as shown in the table below as a ͟Tab ͟Separated ͟Value (TSV) file. This file can be imported into a spreadsheet application.
Full length FASTA
Full length sequences in FASTA format.
Functional Domain FASTA
Sequences of the Functional Domain in FASTA format.
Complete annotation
Download complete annotation of sequences sets of this superfamily as a ͟Tab ͟Separated ͟Value (TSV) file. This file has all data but cell size can exceed the maximum supported by spreadsheet programs (such as Microsoft Excel ®).
Spreadsheet ready annotation
Annotation of sequences sets of this superfamily in a ͟Tab ͟Separated ͟Value (TSV) file. This file can be imported into a spreadsheet application. Cells which exceed the allowed spreadsheet maximum (32.5K) are preceded by the word "Truncated" and clipped short.
Total number of functional domains in this group.
Number of Functional Domains that have been manually or automatically been assigned to a family.
Number of Functional Domains that have not been assigned to a family.
Number of structures available from the PDB for members of this group.
Number of Functional Domains with 100% of Conserved Residues
Number of Functional Domains with less than 100% Conserved Residues
Subgroup ▸ Legend T K C U S
enolase 13877 13530 13303 347 70
┗ enolase 13530 13530 13303 60
galactarate dehydratase 25 2 2 23 6
┗ galactarate dehydratase 2 2 2 2 6
glucarate dehydratase 4012 3585 3585 427 30
┗ glucarate dehydratase 3585 3585 3585 22
mandelate racemase 12476 5059 5043 7417 117
┗ 3,6-anhydro-alpha-L-galactonate cycloisomerase 108 108 108 0
┗ D-arabinonate dehydratase 10 10 10 0
┗ D-galactonate dehydratase 1581 1581 1577 2
┗ D-tartrate dehydratase 290 290 290 3
┗ galactarate dehydratase 3 96 96 96 0
┗ galactarolactone cycloisomerase 53 53 53 0
┗ gluconate dehydratase 13 13 13 0
┗ L-fuconate dehydratase 1191 1191 1191 7
┗ L-galactonate dehydratase 229 229 227 0
┗ L-lyxonate dehydratase 245 245 245 0
┗ L-talarate/galactarate dehydratase 319 319 318 5
┗ mandelate racemase 11 11 7 11
┗ rhamnonate dehydratase 769 769 768 11
┗ xylonate dehydratase 1 10 10 10 0
┗ xylonate dehydratase 2 134 134 130 0
mannonate dehydratase 1844 169 167 1675 39
┗ mannonate dehydratase 169 169 167 24
methylaspartate ammonia-lyase 305 154 150 151 6
┗ methylaspartate ammonia-lyase 154 154 150 6
muconate cycloisomerase 13429 9437 9426 3992 103
┗ 4R-hydroxyproline betaine 2-epimerase 79 79 78 0
┗ cis-3-hydroxy-L-proline dehydratase 29 29 29 0
┗ dipeptide epimerase 4657 4657 4652 29
┗ muconate cycloisomerase (anti) 113 113 113 3
┗ N-succinylamino acid racemase 17 17 17 9
┗ N-succinylamino acid racemase 2 237 237 236 3
┗ o-succinylbenzoate synthase 4305 4305 4301 27
┗ 1: (chloro)muconate cycloisomerase (syn) like 1527 0 0 1527 13
Depth of the multi-level Subgroup hierarchy.