Top Level Name

  ⌊ Superfamily (core) Glutathione Transferase (cytosolic)

    ⌊ Subgroup Main (cytGST)

Total 100% <100% Family unknown
Functional domains 8806
UniProtKB 2424 0 0 2424
GI 4010 0 0 4010
Structures 198
Reactions 0
Functional domains of this subgroup were last updated on June 27, 2017
New functional domains were last added to this subgroup on Oct. 9, 2012

The Main subgroup of cytosolic GSTs (cytGSTs) is a large and diverse class of cytGSTs that encompasses the phi, tau, theta, HSP26, zeta, omega, and beta cytGSTs. While most of these GSTs are bacterial, this group includes large numbers of fungi, animal, and plant GSTs as well. Some of these members use a catalytic serine to activate glutathione, and others use a cysteine. The cysteine of omega class GSTs is in the same position relative to the fold as the serines of phi, tau, theta, HSP26, and zeta classes, while the beta GSTs have a cysteine two positions later. A minority of Main subgroup cytGSTs (including E. coli YfcG) apparently use a threonine to activate glutathione.

Armstrong RN

Structure, catalytic mechanism, and evolution of the glutathione transferases

▸ Abstract

Chem Res Toxicol 1997;10(1):2-18 | PubMed ID: 9074797

Jemth P, Mannervik B

Active site serine promotes stabilization of the reactive glutathione thiolate in rat glutathione transferase T2-2. Evidence against proposed sulfatase activity of the corresponding human enzyme

▸ Abstract

J Biol Chem 2000;275(12):8618-8624 | PubMed ID: 10722701

Copley SD

Evolution of a metabolic pathway for degradation of a toxic xenobiotic: the patchwork approach

▸ Abstract

Trends Biochem Sci 2000;25(6):261-265 | PubMed ID: 10838562

Sheehan D, Meade G, Foley VM, Dowd CA

Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily

▸ Abstract

Biochem J 2001;360(None):1-16 | PubMed ID: 11695986

Wadington MC, Ladner JE, Stourman NV, Harp JM, Armstrong RN

Analysis of the structure and function of YfcG from Escherichia coli reveals an efficient and unique disulfide bond reductase

▸ Abstract

Biochemistry 2009;48(28):6559-6561 | PubMed ID: 19537707

No notes.

Sequence Similarity Networks

Download a Sequence Similarity Network of this subgroup (XGMML format ).

Network downloads are XGMML files that are readable by program such as Cytoscape. In these networks, nodes represent proteins and edges represent pairwise similarities better than a given edge-score cutoff. The edge score is either a bit score for full networks or mean E values for a Repnet. Additionally, these networks contain several attributes with data from the SFLD.

No edge data is available for this network. Contact an SFLD curator to request this network.

List of files included in the download. A detailed list of included node attributes, their definitions, and their uses [revised: 1/24/2014].

Although the download speed has improved please keep in mind that network files can be quite large. We are currently working on improving the network download and finding ways to make large networks manageable. Please see How to increase memory for Cytoscape.

Multiple Sequence Alignment

View the alignment of a representative set of sequences of this subgroup using

Multiple Sequence Alignment

Align one or multiple sequences to the alignment of a representative set of sequences of this subgroup.
Enter FASTA formatted sequence(s) : and view results using


Data Type All (#) Known (#) Unknown (#)
Full length FASTA (8806) (0) (8806)
Complete annotation (.tsv) (8806) (0) (8806)
Annotation suitable for Excel ® (.tsv) (8806) (0) (8806)
Clear form

Some of these files can be quite large, please be patient during the download.

To identify your sequence later, please make sure to provide a header line starting with '>' for each sequence. Empty headers are allowed, but a header line is always required.
Cutoff Value
The least significant edge-score at which pairwise similarities are included in the network. A bit score for the full network, or a mean E value for the Repnet.
XGMML format
Open in Cytoscape via:
 →Network (multiple file types)
Download the annotation of all sequences as shown in the table below as a ͟Tab ͟Separated ͟Value (TSV) file. This file can be imported into a spreadsheet application.
Full length FASTA
Full length sequences in FASTA format.
Functional Domain FASTA
Sequences of the Functional Domain in FASTA format.
Complete annotation
Download complete annotation of sequences sets of this superfamily as a ͟Tab ͟Separated ͟Value (TSV) file. This file has all data but cell size can exceed the maximum supported by spreadsheet programs (such as Microsoft Excel ®).
Spreadsheet ready annotation
Annotation of sequences sets of this superfamily in a ͟Tab ͟Separated ͟Value (TSV) file. This file can be imported into a spreadsheet application. Cells which exceed the allowed spreadsheet maximum (32.5K) are preceded by the word "Truncated" and clipped short.
Total number of functional domains in this group.
Number of Functional Domains that have been manually or automatically been assigned to a family.
Number of Functional Domains that have not been assigned to a family.
Number of structures available from the PDB for members of this group.
Number of Functional Domains with 100% of Conserved Residues
Number of Functional Domains with less than 100% Conserved Residues
Depth of the multi-level Subgroup hierarchy.