Reaction: [formate-C-acetyltransferase]-activating enzyme List of reactions

Reaction name: [formate-C-acetyltransferase]-activating enzyme Reaction type: cognate
EC number:1.97.1.4 Kegg ID:R04710 Rhea ID:
Directionality: reversible Curation status: valid Classification: public
Description:A single glycine residue in EC 2.3.1.54, formate C-acetyltransferase, is oxidized to the corresponding radical by transfer of H from its CH2 to S-adenosyl-L-methionine (AdoMet) with concomitant cleavage of the latter.
Curator notes:This reaction is missing a structure for the semi-quinone product, therefore will not chemically balance at this time.
ID Type Compound ID Name Stoichiometry Curator notes
chebi 67040 S-adenosyl-L-methioninate 1
pdb 2pfl Pyruvate Formate-Lyase (inactive) 1 Specifically, the Gly residue that is to become an activated glycyl radical.
chebi 62787 1,5-dihydroflavin 1
ID Type Compound ID Name Stoichiometry Curator notes
chebi 57844 L-methionine zwitterion 1
chebi 17319 5'-deoxyadenosine 1
chebi 15817 semiquinone 1
pdb 2pfl Pyruvate Formate-Lyase (active) 1 The PFL protein is now in its activated glycyl form.

Reaction

[formate-C-acetyltransferase]-activating enzyme

+ + + + +
S-adenosyl-L-methioninate
67040
Pyruvate Formate-Lyase (inactive)
2pfl
1,5-dihydroflavin
62787
L-methionine zwitterion
57844
5'-deoxyadenosine
17319
semiquinone
15817
Pyruvate Formate-Lyase (active)
2pfl

EC: 1.97.1.4 | IntEnz: 1.97.1.4 | Kegg: 1.97.1.4 | BioCyc: 1.97.1.4 | BRENDA: 1.97.1.4